Axonal spiking patterns during high-frequency firing

高频放电期间的轴突尖峰模式

• 批准号: 6878541
• 负责人: ZAYD M KHALIQ
• 金额: $ 4.18万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6878541
• 关键词: action potentials; axon; biological signal transduction; calcium indicator; cellular polarity; cerebellar Purkinje cell; cerebellar nuclei; electrophysiology; laboratory mouse; neural information processing; neural transmission; neurophysiology; predoctoral investigator

DESCRIPTION (provided by applicant): As the sole output from the cerebellar cortex, Purkinje cells are responsible for processing information from diverse brain regions and delivering this information to the cells of the cerebellar nucleus. Although signal processing is known to occur in the dendrites and the soma, little is known about the processing that may also occur in the axons of Purkinje cells. Specifically, we are interested in the pattern of axonal action potentials that result from the massive depolarization observed in Purkinje cell somata and dendrites during stimulation of climbing fibers (i.e. complex spike). Moreover, high-frequency firing and extensive branching may reduce the reliability of transmission in axons. Using electrophysiological and imaging techniques, we propose to determine the transfer function of Purkinje cell axons in periods of high-frequency and burst firing. We also propose to test the reliability of action potential propagation throughout the highly ramifying collateral axons and terminal projections to the deep cerebellar nucleus.

描述（由申请人提供）：作为小脑皮质的唯一输出，浦肯野细胞负责处理来自不同脑区的信息并将此信息传递给小脑核细胞。虽然已知信号处理发生在树突和索马中，但对浦肯野细胞轴突中也可能发生的信号处理知之甚少。具体而言，我们感兴趣的轴突动作电位的模式，从浦肯野细胞胞体和树突中观察到的大量去极化过程中的刺激攀爬纤维（即复杂的穗）。此外，高频放电和广泛的分支可能会降低轴突中传输的可靠性。使用电生理和成像技术，我们建议确定传递函数的浦肯野细胞轴突在高频率和突发放电的时期。我们还建议测试的可靠性，整个高度分歧侧支轴突和终端预测小脑深核的动作电位传播。