Core--Animal Handling and Imaging

描述（由申请人提供）：动物处理和成像核心的主要目标有五个方面：1）购买、创建或获取项目项目所需的小鼠遗传模型，并在受控育种项目下维护供两个或多个项目使用的小鼠（“核心”小鼠）。2)根据要求向研究者提供CORE小鼠，以完成项目特定实验。3)为乳腺特异性和一般外科手术提供最先进的设施和技术支持以及专业知识。4)作为PPG研究者之间组织共享的协调中心。5)为评价活体小鼠中肿瘤的发展和转移提供成像能力。此外，该核心将是 负责所有与项目相关的日常费用。由于动物设施的空间是有限的，每个项目都分配了一定数量的笼子，这些笼子可以在任何给定的时间平均维持。最后，动物处理核心将提供项目生成的所有小鼠的数据库以及与组织样本、基因表达谱和组织学评价相关的文件。数据输入和数据库格式将基于我们广泛的人体组织库的现有软件。项目之间的组织共享和动物空间协调将导致具体的成本节约，除了提高共享的专业知识和设施（如成像设备）的有效性之外。该项目中的所有五个项目都需要动物处理和成像核心。

DESCRIPTION (provided by applicant): The primary objectives of the Animal Handling and Imaging Core are five-fold: 1) To purchase, create, or acquire mouse genetic models required for the program project and to maintain those to be used by two or more projects ("CORE" mice) under a controlled breeding program. 2) To provide CORE mice to investigators for completion of project-specific experiments upon request. 3) To provide state-of-the-art facilities and technical support for, and expertise in, mammary-specific and general surgical procedures. 4) To serve as a center for coordination of tissue sharing among PPG investigators. 5) To provide imaging capabilities for evaluation of tumor development and metastasis in living mice. In addition, this Core will be responsible for all per diem cage costs related to the program project. Because space in the animal facility is finite, each project has been allocated a certain number of cages that can be maintained on average at any given time. Finally, the Animal Handling Core will provide a database of all mice generated by the project and documentation related to tissue samples, gene expression profiles, and histological evaluation. The data entry and database format will be based on our existing software for our extensive human tissue banks. Tissue sharing and animal space coordination among the Projects will result in specific cost savings, in addition to the enhanced effectiveness of shared expertise and facilities such as the imaging equipment. The Animal Handling and Imaging Core is required by all five Projects in the Program Project.