Clinical Information

临床信息

• 批准号: 6728860
• 负责人: ELIZABETH A WAYNER
• 金额: $ 29.12万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-12 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6728860
• 关键词: biomedical facility; bioreactors; green fluorescent proteins; hybridomas; immunologic substance development /preparation; molecular cloning; monoclonal antibody; peptide library

The Antibody Development Facility provides investigators with custom designed monoclonal antibodies. In most, if not all cases, these are necessary reagents that are not commercially available. Services provided include: a) the design of antigens, immunization and screening strategies b) hybridoma cell line production via traditional methods, c) optimization of existing hybridoma cell lines by selection cloning d) production of monoclonal antibodies in species other than mice, e) services to wean hybridoma cell lines off selection media and into a variety of basal and serum free media for use in mini-bioreactors, immune precipitation or in vivo studies, f) cloning, expression and engineering of single chain antibody variable fragments (scFvs) or scFvs containing biological reporter molecules such as green fluorescent protein and g) the selection of antigen specific antibodies and their fragments from phage display libraries. This application requests continued support for a resource which continues to fulfill an essential role for peer reviewed research.

抗体开发设施为研究人员提供定制设计的单克隆抗体 抗体。在大多数（如果不是全部）情况下，这些都是必需的试剂，但无法市售。 提供的服务包括：a) 抗原设计、免疫和筛选策略 b) 通过传统方法生产杂交瘤细胞系，c) 通过选择克隆优化现有杂交瘤细胞系 d) 在小鼠以外的物种中生产单克隆抗体，e) 使杂交瘤细胞系脱离选择培养基并进入各种基础和无血清培养基的服务，用于微型生物反应器、免疫沉淀或 体内研究， f) 单链抗体可变片段（scFv）或含有生物报告分子（例如绿色荧光蛋白）的 scFv 的克隆、表达和工程化，以及 g) 从噬菌体展示文库中选择抗原特异性抗体及其片段。该申请要求继续支持资源，该资源继续在同行评审研究中发挥重要作用。