Neuronal and Hematopoietic Gene Regulation of GATA-2

GATA-2 的神经元和造血基因调控

• 批准号: 6640691
• 负责人: William Todd Penberthy
• 金额: $ 2.97万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-12 至 2004-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6640691
• 关键词: artificial chromosomes; cell proliferation; complementary DNA; developmental genetics; disease /disorder model; functional /structural genomics; gene expression; genetic library; genetic mapping; genetic promoter element; genetic regulation; genetic regulatory element; genetically modified animals; green fluorescent proteins; hematopoiesis; hematopoietic stem cells; neurogenesis; neurons; protein structure function; transcription factor; zebrafish

DESCRIPTION: (Investigator's abstract) Analysis of living transgenic zebrafish expressing the green fluorescent protein driven by regulatory promoter sequences allows rapid identification of distant upstream specific cis-acting elements required for normal expression of developmentally essential genes. The GATA-2 gene is expressed in both hematopoietic and neuronal tissues, and has been shown to play an essential role in maintenance and proliferation of hematopoietic cells. However, the mechanisms underlying the regulation of GATA-2 gene expression and its function in the nervous system is unknown. We have generated artificial chromosome transgenic constructs that can recapitulate the endogenous expression pattern of GATA-2 in zebrafish, and have already characterized a cis-acting element required for its neuronal expression. This proposal aims to identify and characterize the genes that function to confer neuronal and hematopoietic expression of the GATA-2 gene. The outcome should make a contribution to the developmental biology of both hematopoiesis and the neurogenesis.

描述：（研究者摘要）活体转基因斑马鱼的分析 表达由调控启动子驱动的绿色荧光蛋白 序列允许快速鉴定远端上游特异性顺式作用 发育必需基因正常表达所需的元素。的 加塔-2基因在造血和神经组织中表达，并且具有 已被证明在维持和扩散中起着至关重要的作用， 造血细胞然而，调控的机制 加塔-2基因的表达及其在神经系统中的功能尚不清楚。我们 已经产生了人工染色体转基因构建体， 概括了加塔-2在斑马鱼中的内源性表达模式， 已经描述了其神经元所需的顺式作用元件， 表情该提案旨在识别和描述基因， 具有赋予加塔-2基因的神经元和造血表达的功能。 这一结果应该对两者的发育生物学做出贡献。 造血和神经发生。