Neuronal and Hematopoietic Gene Regulation of GATA-2

描述：(调查摘要)对活的转基因斑马鱼的分析 调控启动子驱动的绿色荧光蛋白的表达 序列可以快速识别远距离上游特定的顺式作用 发育必需基因正常表达所需的元件。这个 GATA-2基因在造血和神经组织中均有表达，并具有 已被证明在维持和扩散 造血细胞。然而，监管的基础机制是 GATA-2基因在神经系统中的表达和功能尚不清楚。我们 已经产生了人造染色体转基因结构，可以 概述了GATA-2在斑马鱼中的内源表达模式，并有 已经表征了其神经元所需的顺式作用元件 表情。这项提议旨在识别和表征 GATA-2基因在神经细胞和造血细胞中的表达。 这一结果应该对两者的发育生物学做出贡献。 造血和神经发生。

DESCRIPTION: (Investigator's abstract) Analysis of living transgenic zebrafish expressing the green fluorescent protein driven by regulatory promoter sequences allows rapid identification of distant upstream specific cis-acting elements required for normal expression of developmentally essential genes. The GATA-2 gene is expressed in both hematopoietic and neuronal tissues, and has been shown to play an essential role in maintenance and proliferation of hematopoietic cells. However, the mechanisms underlying the regulation of GATA-2 gene expression and its function in the nervous system is unknown. We have generated artificial chromosome transgenic constructs that can recapitulate the endogenous expression pattern of GATA-2 in zebrafish, and have already characterized a cis-acting element required for its neuronal expression. This proposal aims to identify and characterize the genes that function to confer neuronal and hematopoietic expression of the GATA-2 gene. The outcome should make a contribution to the developmental biology of both hematopoiesis and the neurogenesis.