CORE--BIOMOLECULAR RESEARCH CORE

糖尿病和内分泌研究中心（DERC）的生物分子研究核心的主要目标是在蛋白质和DNA科学领域提供高质量，具有成本效益，复杂的分析和合成服务，仪器和咨询。为了实现这些目标，我们提供以下具体服务：蛋白质科学-氨基酸分析；Edman和质谱多肽测序和磷酸肽测序；蛋白质和肽的质谱分析和翻译后蛋白质修饰的测定；CD spectropoladmetry;动态光散射；大、小规模多肽合成及抗多肽抗原生产。蛋白质组学分析采用二维PAGE和正交色谱结合纳米喷雾i/ms/ms。

The primary objectives of the Biomolecular Research Core of the Diabetes and Endocrinology Research Center (DERC) are to provide high quality, cost-effective, sophisticated analytical and synthetic services, instrumentation and consultation in the areas of protein, and DNA sciences. To achieve these goals the following specific services are offered: Protein Sciences - Amino acid analysis; Edman and mass spectrometric peptide sequencing and phosphopeptide sequencing; Mass spectrometric protein and peptide analysis and determination of post-translational protein modifications; CD spectropoladmetry; Dynamic light scattering; Large and small-scale peptide synthesis and anti-peptide antigen production. Proteomic analyses using 2D PAGE and orthogonai chromatography coupled with nanospray i/ms/ms. DNA Sciences - Automated DNA sequencing, genotyping and satellite mapping; DNA chemical manipulation. Affymetrix GeneChip and custom microarray gene profiling. Quantitative RT-PCR. High throughput oligonucleotide synthesis and "boutique" oligonucleotide synthesis. Expert consultation on the planning and implementation of research projects and individual experiments. These services are provided using either core personnel or the use of core instrumentation by trained investigators and students. Particular emphasis in the core is directed at the development of technologies and protocols which are in demand by investigators in the DERC. Recent expansion in the core includes a 2D gel electrophoresis service for sample preparation for mass spectrometric sequencing of proteins (proteomics), new mass spectrometers for ultra-high sensitivity protein sequence analysis, high throughput capillary DNA sequencer, high throughput oligonucleotide synthesizer; and a quantitative RT-PCR instrument for gene chip target validation and real-time PCR.