CORE--BIOMOLECULAR RESEARCH CORE

糖尿病和内分泌学研究中心（DERC）生物分子研究核心的主要目标是在蛋白质和DNA科学领域提供高质量，具有成本效益，复杂的分析和合成服务，仪器和咨询。为了实现这些目标，提供以下具体服务：蛋白质科学-氨基酸分析; Edman和质谱肽测序和磷酸肽测序;质谱蛋白质和肽分析和翻译后蛋白质修饰的测定; CD光谱法;动态光散射;大规模和小规模肽合成和抗肽抗原生产。使用2D PAGE和正交色谱法结合nanospray i/ms/ms进行蛋白质组学分析。 DNA科学-自动DNA测序，基因分型和卫星定位; DNA化学 操纵Affyscore基因芯片和定制微阵列基因分析。定量RT-PCR。高通量寡核苷酸合成和“精品”寡核苷酸合成。就研究项目和个别实验的规划和执行提供专家咨询。 这些服务由核心人员提供，或由经过培训的调查人员和学生使用核心仪器提供。核心部分特别强调的是DERC研究人员所需的技术和协议的开发。最近的核心扩展包括用于蛋白质质谱测序（蛋白质组学）样品制备的2D凝胶电泳服务，用于超高灵敏度蛋白质序列分析的新型质谱仪，高通量毛细管DNA测序仪，高通量寡核苷酸合成仪;以及用于基因芯片靶标验证和实时PCR的定量RT-PCR仪器。

The primary objectives of the Biomolecular Research Core of the Diabetes and Endocrinology Research Center (DERC) are to provide high quality, cost-effective, sophisticated analytical and synthetic services, instrumentation and consultation in the areas of protein, and DNA sciences. To achieve these goals the following specific services are offered: Protein Sciences - Amino acid analysis; Edman and mass spectrometric peptide sequencing and phosphopeptide sequencing; Mass spectrometric protein and peptide analysis and determination of post-translational protein modifications; CD spectropoladmetry; Dynamic light scattering; Large and small-scale peptide synthesis and anti-peptide antigen production. Proteomic analyses using 2D PAGE and orthogonai chromatography coupled with nanospray i/ms/ms. DNA Sciences - Automated DNA sequencing, genotyping and satellite mapping; DNA chemical manipulation. Affymetrix GeneChip and custom microarray gene profiling. Quantitative RT-PCR. High throughput oligonucleotide synthesis and "boutique" oligonucleotide synthesis. Expert consultation on the planning and implementation of research projects and individual experiments. These services are provided using either core personnel or the use of core instrumentation by trained investigators and students. Particular emphasis in the core is directed at the development of technologies and protocols which are in demand by investigators in the DERC. Recent expansion in the core includes a 2D gel electrophoresis service for sample preparation for mass spectrometric sequencing of proteins (proteomics), new mass spectrometers for ultra-high sensitivity protein sequence analysis, high throughput capillary DNA sequencer, high throughput oligonucleotide synthesizer; and a quantitative RT-PCR instrument for gene chip target validation and real-time PCR.