QUANTATIVE AND PHOSPHO PROTEOMICS INSTRUMENTATION

定量和磷酸化蛋白质组学仪器

• 批准号: 7335276
• 负责人: Jay William FOX
• 金额: $ 47.66万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335276
• 关键词: biomedical facility; dogs; proteins; proteomics; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): In this proposal, we are requesting support for the purchase of a mass spectrometer to perform experiments involving identification and quantification of protein expression levels and post-translational modifications (PTMs). Specifically, we are requesting a hybrid quadrupole time-of-flight mass spectrometer system (QSTAR XL) from Applied Biosystems. The abilities of this instrument to accurately measure both peptide masses and fragment ion masses as well as provide complementary MS/MS fragmentation patterns will offer increased certainty in the analysis of PTMs found in protein samples. In addition, the system has been engineered to facilitate not only the identification, but also the quantification of proteins within a given sample using novel iTRAQ reagents. As the field of proteomics moves beyond the level of simple identification into the more challenging realm of changes in protein structure and expression, it is vital that we acquire instrumentation which will allow our investigators to probe these effects. This instrument will be housed in the University of Virginia Biomolecular Research Facility and will support the research proteomics projects of eight NIH-funded investigators. For these projects, we anticipate that the instrument will be used approximately 80% of the available time. The remaining time will be made available for new proteomic project development. Dr. Jay Fox, Assistant Dean for Research, will be responsible for the overall management of the instrumentation. Dr. Nicholas Sherman will be responsible for interacting with investigators concerning the planning and execution of experiments and data interpretation. Dr. Kristina Nelson will be responsible for daily operation and maintenance of the instrument as well as data analysis. The Biomolecular Research Facility has been providing mass spectrometric expertise for the past 11 years, and we expect seamless integration of the requested system with current instrumentation. A fee-for-service charge will be instituted to help defray the costs of staff salaries and supplies. The oversight of the Biomolecular Research Facility is provided by a standing committee appointed by the Dean of the Medical School. The Oversight Committee provides guidance and expertise directly to Dr. Fox for the management of the facility as well as complementing the scientific expertise of facility staff. The University of Virginia Biomolecular Research Facility has been in operation for the past 24 years with continual fiscal support by the Health Sciences Center. The support by the administration, along with the operational plan, ensures adequate and appropriate instrument use for the fiscally sound and scientifically successful operation of instrumentation requested for the benefit of NIH-funded investigators at UVa.

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：在这项提案中，我们请求支持购买一台质谱仪，以执行涉及识别和量化蛋白质表达水平以及翻译后修饰(PTM)的实验。具体地说，我们要求应用生物系统公司提供一种混合式四极飞行时间质谱仪系统(QSTAR XL)。该仪器能够准确测量多肽质量和碎片离子质量，并提供互补的MS/MS裂解模式，将为蛋白质样品中发现的PTMS的分析提供更多的确定性。此外，该系统的设计不仅有助于鉴定，还有助于使用新型iTRAQ试剂对给定样本中的蛋白质进行定量。随着蛋白质组学领域超越简单的鉴定水平，进入更具挑战性的蛋白质结构和表达变化领域，我们必须获得使我们的研究人员能够探索这些影响的仪器。该仪器将被安置在弗吉尼亚大学生物分子研究设施中，并将支持八名由NIH资助的研究人员的蛋白质组学研究项目。对于这些项目，我们预计仪器将使用大约80%的可用时间。剩余的时间将用于新的蛋白质组项目开发。负责研究的助理院长Jay Fox博士将负责仪器的整体管理。尼古拉斯·谢尔曼博士将负责与调查人员就计划和执行实验以及数据解释进行互动。克里斯蒂娜·纳尔逊博士将负责仪器的日常操作和维护以及数据分析。生物分子研究设施在过去的11年里一直提供质谱学专业知识，我们希望所要求的系统与当前的仪器无缝集成。将实行按服务收费，以帮助支付工作人员工资和用品费用。生物分子研究设施的监督由医学院院长任命的一个常设委员会提供。监督委员会直接向福克斯博士提供指导和专门知识，以管理该设施，并补充设施工作人员的科学专长。弗吉尼亚大学生物分子研究设施在过去24年里一直在健康科学中心的财政支持下运行。行政部门的支持，以及业务计划，确保充分和适当的仪器使用，以财政健全和科学上成功的仪器操作所需的国家卫生研究所资助的调查人员在弗吉尼亚大学。