Quantative and Phospho Proteomics Instrumentation

描述（由申请人提供）：在本提案中，我们请求支持购买质谱仪，以进行涉及蛋白质表达水平和翻译后修饰（PTM）鉴定和定量的实验。具体而言，我们正在请求应用生物系统公司提供混合四极杆飞行时间质谱仪系统（QSTAR XL）。该仪器准确测量肽质量和碎片离子质量以及提供互补MS/MS碎片模式的能力将为蛋白质样品中发现的PTM分析提供更高的确定性。此外，该系统经过改造，不仅有助于识别，而且还有助于使用新型iTRAQ试剂定量给定样品中的蛋白质。随着蛋白质组学领域超越简单鉴定的水平，进入蛋白质结构和表达变化的更具挑战性的领域，我们获得能够让我们的研究人员探测这些影响的仪器是至关重要的。该仪器将被安置在弗吉尼亚大学生物分子研究设施，并将支持八个NIH资助的研究人员的研究蛋白质组学项目。对于这些项目，我们预计该仪器将使用约80%的可用时间。剩余时间将用于新的蛋白质组学项目开发。 博士杰伊福克斯，研究助理院长，将负责仪器的整体管理。Nicholas谢尔曼博士将负责与研究者就实验的计划和执行以及数据解释进行互动。Dr. Kristina纳尔逊将负责仪器的日常操作和维护以及数据分析。生物分子研究设施在过去的11年里一直提供质谱专业知识，我们希望所要求的系统与当前的仪器无缝集成。将收取服务费，以帮助支付工作人员薪金和用品费用。 生物分子研究设施的监督由医学院院长任命的常务委员会提供。监督委员会直接向Fox博士提供设施管理方面的指导和专业知识，并补充设施工作人员的科学专业知识。弗吉尼亚大学生物分子研究设施在过去的24年里一直在运作，并得到健康科学中心的持续财政支持。行政部门的支持，沿着的操作计划，确保充分和适当的仪器使用的仪器要求的利益NIH资助的研究人员在弗吉尼亚大学的健康和科学上成功的操作。

DESCRIPTION (provided by applicant): In this proposal, we are requesting support for the purchase of a mass spectrometer to perform experiments involving identification and quantification of protein expression levels and post-translational modifications (PTMs). Specifically, we are requesting a hybrid quadrupole time-of-flight mass spectrometer system (QSTAR XL) from Applied Biosystems. The abilities of this instrument to accurately measure both peptide masses and fragment ion masses as well as provide complementary MS/MS fragmentation patterns will offer increased certainty in the analysis of PTMs found in protein samples. In addition, the system has been engineered to facilitate not only the identification, but also the quantification of proteins within a given sample using novel iTRAQ reagents. As the field of proteomics moves beyond the level of simple identification into the more challenging realm of changes in protein structure and expression, it is vital that we acquire instrumentation which will allow our investigators to probe these effects. This instrument will be housed in the University of Virginia Biomolecular Research Facility and will support the research proteomics projects of eight NIH-funded investigators. For these projects, we anticipate that the instrument will be used approximately 80% of the available time. The remaining time will be made available for new proteomic project development. Dr. Jay Fox, Assistant Dean for Research, will be responsible for the overall management of the instrumentation. Dr. Nicholas Sherman will be responsible for interacting with investigators concerning the planning and execution of experiments and data interpretation. Dr. Kristina Nelson will be responsible for daily operation and maintenance of the instrument as well as data analysis. The Biomolecular Research Facility has been providing mass spectrometric expertise for the past 11 years, and we expect seamless integration of the requested system with current instrumentation. A fee-for-service charge will be instituted to help defray the costs of staff salaries and supplies. The oversight of the Biomolecular Research Facility is provided by a standing committee appointed by the Dean of the Medical School. The Oversight Committee provides guidance and expertise directly to Dr. Fox for the management of the facility as well as complementing the scientific expertise of facility staff. The University of Virginia Biomolecular Research Facility has been in operation for the past 24 years with continual fiscal support by the Health Sciences Center. The support by the administration, along with the operational plan, ensures adequate and appropriate instrument use for the fiscally sound and scientifically successful operation of instrumentation requested for the benefit of NIH-funded investigators at UVa.