PROTEOMICS

蛋白质组学

• 批准号: 7313419
• 负责人: Jay William FOX
• 金额: $ 13万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-10 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7313419
• 关键词: Drosophilidae; affinity chromatography; biomedical facility; cell migration; cooperative study; functional /structural genomics; green fluorescent proteins; informatics; integrins; intermolecular interaction; mass spectrometry; phosphorylation; protein purification; protein structure function; proteins; proteomics; reporter genes

The overall goal of the Proteomics Initiative is to use mass spectrometry based proteomic methods to expand the current migration knowledge database by examining phosphorylation site utilization of proteins that regulate cell motility, by further developing methods for measuring differential phosphorylation of those proteins, and by using quantitative methods to assess positional and kinetic variation in phosphorylation site utilization in migration-related proteins. In addition, the Initiative will extend these proteomic methods to provide positional cartography of the proteins present in protein complexes formed by known migration-related proteins and protein products of novel migration genes identified in the unbiased screens of the Gene Discovery Initiative. These goals are enabled by several pivotal developments in technology, analysis, and methodology carried out during the first phase of funding. The Initiative focuses on two inter-related themes: 1. Phosphoproteomics of migration related proteins and 2. Positional proteomics of migration-related protein complexes and their phoshorylations.

蛋白质组学计划的总体目标是使用基于蛋白质组学的质谱学 方法通过研究磷酸化位点的利用来扩展现有的迁移知识库 调节细胞运动的蛋白质，通过进一步发展测量差异磷酸化的方法 并通过使用定量方法来评估磷酸化的位置和动力学变化 迁移相关蛋白中的位点利用。此外，该倡议将把这些蛋白质组学方法扩展到 提供已知迁移相关形成的蛋白质复合体中存在的蛋白质的位置地图 在基因无偏筛选中发现的新迁移基因的蛋白质和蛋白质产物 探索计划。这些目标是由技术、分析和技术方面的几项关键发展促成的 在第一阶段筹资期间采用的方法。该倡议侧重于两个相互关联的主题：1. 迁移相关蛋白的磷酸蛋白质组学和2.迁移相关蛋白的位置蛋白质组学 络合物及其光酰化反应。