NA, K PUMP IN AGING SKELETAL MUSCLE

描述（改编自申请人摘要）：骨骼肌功能障碍 在老年人中具有深远的临床意义。的重点 应用是阐明病理生理适应的机制 骨骼肌的衰老，并评估叠加的影响， 练习这些适应性。四个具体目标概述：1。阐明 与年龄相关的Na-K ATP酶表达和活性改变。 这项生化评估将着眼于肌群特异性，亚细胞 分布、亚基缔合以及翻译前和翻译后机制 的修改。2.为了评估骨骼肌中的体力活动是否 可以减弱或逆转这些不同特征中与年龄相关的变化 Na-K ATP酶的活性。3.为了探索衰老是否与改变的 钾平衡，这将反映改变的钠钾泵功能。4. 为了验证体力活动会改善钾平衡的假设， 骨骼肌在衰老过程中的作用。应用程序将使用一个 各种生化技术和微透析技术来测试组织 钾通量

DESCRIPTION (Adapted from the applicant's abstract):Skeletal muscle dysfunction is of profound clinical significance in the elderly. The focus of this application is to elucidate mechanisms underlying pathophysiologic adaptation of skeletal muscle to aging and to evaluate the effects of superimposed exercise on these adaptations. Four specific aims are outlined: 1. To elucidate age associated alterations in the expression and activity of the Na-K ATPase. This biochemical evaluation will look at muscle group specificity, subcellular distribution, sub unit association, and pre and post translational mechanisms of modification. 2. To evaluate whether physical activity in skeletal muscle can attenuate or reverse age associated changes in the these various features of Na-K ATPase. 3. To explore whether or not aging is associated with altered potassium balance which would be reflective of altered Na-K pump function. 4. To test the hypothesis that physical activity will improve potassium balance in skeletal muscle during the process of aging. The application will make use of a variety of biochemical techniques and also microdialysis to test tissue potassium fluxes.