NA, K PUMP IN AGING SKELETAL MUSCLE

NA、K 泵送老化骨骼肌

• 批准号: 6942582
• 负责人: YUK-CHOW NG
• 金额: $ 24.59万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6942582
• 关键词: aging; enzyme activity; enzyme induction /repression; exercise; isozymes; laboratory rat; microdialysis; muscle function; potassium; sodium potassium exchanging ATPase; striated muscles

DESCRIPTION (Adapted from the applicant's abstract):Skeletal muscle dysfunction is of profound clinical significance in the elderly. The focus of this application is to elucidate mechanisms underlying pathophysiologic adaptation of skeletal muscle to aging and to evaluate the effects of superimposed exercise on these adaptations. Four specific aims are outlined: 1. To elucidate age associated alterations in the expression and activity of the Na-K ATPase. This biochemical evaluation will look at muscle group specificity, subcellular distribution, sub unit association, and pre and post translational mechanisms of modification. 2. To evaluate whether physical activity in skeletal muscle can attenuate or reverse age associated changes in the these various features of Na-K ATPase. 3. To explore whether or not aging is associated with altered potassium balance which would be reflective of altered Na-K pump function. 4. To test the hypothesis that physical activity will improve potassium balance in skeletal muscle during the process of aging. The application will make use of a variety of biochemical techniques and also microdialysis to test tissue potassium fluxes.

描述（改编自申请人的摘要）：骨骼肌功能障碍 对于老年人来说具有深远的临床意义。本次的重点 应用是为了阐明病理生理适应的机制 骨骼肌对衰老的影响并评估叠加的影响 对这些适应进行练习。概述了四个具体目标： 1. 阐明 Na-K ATP酶的表达和活性与年龄相关的变化。 该生化评估将着眼于肌肉群特异性、亚细胞 分布、子单元关联以及翻译前和翻译后机制 的修改。 2. 评估骨骼肌的体力活动是否 可以减弱或逆转这些不同特征的与年龄相关的变化 Na-K ATP 酶。 3. 探讨衰老是否与改变有关 钾平衡反映了钠钾泵功能的改变。 4. 检验体力活动可改善体内钾平衡的假设 骨骼肌在衰老过程中。该应用程序将利用 各种生化技术以及微透析来测试组织 钾通量。

项目成果

Fiber type-specific immunostaining of the Na+,K+-ATPase subunit isoforms in skeletal muscle: age-associated differential changes.

骨骼肌中 Na,K-ATP 酶亚基亚型的纤维类型特异性免疫染色：年龄相关的差异变化。

• DOI: 10.1016/j.bbadis.2006.08.006
• 发表时间: 2006
• 期刊: Biochimica et biophysica acta
• 作者: Zhang,Lianqin;Morris,KeithJ;Ng,Yuk-Chow
• 通讯作者: Ng,Yuk-Chow