NA, K PUMP IN AGING SKELETAL MUSCLE

描述(改编自申请人的摘要)：骨骼肌功能障碍 对老年人具有深远的临床意义。这件事的重点是 应用于阐明潜在的病理生理适应机制 骨骼肌对衰老的影响及叠加效应的评价 在这些适应上进行锻炼。概述了四个具体目标：1.澄清 Na-K-ATPase的表达和活性与年龄相关的变化。 这项生化评估将着眼于肌肉群的特异性、亚细胞 分布、亚单位关联以及翻译前和翻译后机制 修改的结果。2.评估骨骼肌的体力活动 可以减弱或逆转这些不同特征中与年龄相关的变化 Na-K-ATPase。3.探索衰老是否与改变有关 反映Na-K泵功能改变的钾平衡。4. 为了验证体力活动将改善体内钾平衡的假设 骨骼肌在衰老过程中。该应用程序将使用 各种生化技术和微透析法检测组织 钾离子通量。

DESCRIPTION (Adapted from the applicant's abstract):Skeletal muscle dysfunction is of profound clinical significance in the elderly. The focus of this application is to elucidate mechanisms underlying pathophysiologic adaptation of skeletal muscle to aging and to evaluate the effects of superimposed exercise on these adaptations. Four specific aims are outlined: 1. To elucidate age associated alterations in the expression and activity of the Na-K ATPase. This biochemical evaluation will look at muscle group specificity, subcellular distribution, sub unit association, and pre and post translational mechanisms of modification. 2. To evaluate whether physical activity in skeletal muscle can attenuate or reverse age associated changes in the these various features of Na-K ATPase. 3. To explore whether or not aging is associated with altered potassium balance which would be reflective of altered Na-K pump function. 4. To test the hypothesis that physical activity will improve potassium balance in skeletal muscle during the process of aging. The application will make use of a variety of biochemical techniques and also microdialysis to test tissue potassium fluxes.