Instrumental Control of Ion-Selective Electrodes

离子选择电极的仪器控制

• 批准号: 6811351
• 负责人: ERIC BAKKER
• 金额: $ 19.91万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2005-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6811351
• 关键词: analytical method; biomedical equipment development; chemical stability; coulometry; electrochemistry; electrodes; heparin; ion transport; ionophores; membrane activity; membrane potentials; method development; polyion; protamines

DESCRIPTION (provided by applicant): This research establishes the instrumental control of ion-selective electrodes as an alternative measurement principle to traditional zero current potentiometry. While ion-selective electrodes are routine tools in clinical laboratories all over the world, numerous new sensing principles that have recently been developed for ISEs make use of ion fluxes, for which classical potentiometry is no longer an adequate transduction method. It is proposed to apply repetitive current and potential pulses to ion-selective membranes that are designed to lack ion-exchanger properties. The resulting sensor responses have the same look and feel as in zero current potentiometry and will be comprehensively compared to each other. As an initial important application of this method, fully reversible polyion sensors for the detection of the anticoagulant heparin and its antidote protamine will be developed. For this first time, this methodology forms the foundation for a truly continuous monitoring technology for these important analytes in whole blood samples during surgery. In addition, the interrogation technique will be optimized and applied to numerous other cases where zero current potentiometry has shown, or is expected to show, unsatisfactory sensor behavior.

描述（由申请人提供）：本研究确立了离子选择性电极的仪器控制作为传统零电流电位法的替代测量原理。虽然离子选择性电极是世界各地临床实验室的常规工具，但最近为ISE开发的许多新的传感原理利用了离子通量，经典的电位测定法不再是适当的转导方法。提出了将重复的电流和电势脉冲施加到被设计为缺乏离子交换性能的离子选择性膜。所得到的传感器响应具有与零电流电位法相同的外观和感觉，并且将彼此进行全面比较。作为该方法的一个初步的重要应用，完全可逆的聚离子传感器检测抗凝肝素及其解毒剂鱼精蛋白将被开发。这是第一次，这种方法为手术期间全血样本中这些重要分析物的真正连续监测技术奠定了基础。此外，询问技术将被优化并应用于许多其他情况下，零电流电位法已经显示，或预计将显示，不满意的传感器的行为。