Instrumental Control of Ion-Selective Electrodes

描述（由申请人提供）：本研究建立了离子选择电极的仪器控制，作为传统零电流电位法的替代测量原理。虽然离子选择电极是世界各地临床实验室的常规工具，但最近为ise开发的许多新的传感原理都利用了离子通量，对于离子通量，经典的电位法不再是一种适当的转导方法。本文提出将重复电流和电位脉冲应用于缺乏离子交换特性的离子选择膜。由此产生的传感器响应具有与零电流电位计相同的外观和感觉，并将相互进行全面比较。作为该方法的初步重要应用，将开发用于抗凝血肝素及其解毒剂鱼精蛋白检测的全可逆多离子传感器。这是第一次，该方法为手术期间全血样本中这些重要分析物的真正连续监测技术奠定了基础。此外，审讯技术将被优化并应用于许多其他情况下，其中零电流电位计已经显示，或预计将显示，不满意的传感器行为。

DESCRIPTION (provided by applicant): This research establishes the instrumental control of ion-selective electrodes as an alternative measurement principle to traditional zero current potentiometry. While ion-selective electrodes are routine tools in clinical laboratories all over the world, numerous new sensing principles that have recently been developed for ISEs make use of ion fluxes, for which classical potentiometry is no longer an adequate transduction method. It is proposed to apply repetitive current and potential pulses to ion-selective membranes that are designed to lack ion-exchanger properties. The resulting sensor responses have the same look and feel as in zero current potentiometry and will be comprehensively compared to each other. As an initial important application of this method, fully reversible polyion sensors for the detection of the anticoagulant heparin and its antidote protamine will be developed. For this first time, this methodology forms the foundation for a truly continuous monitoring technology for these important analytes in whole blood samples during surgery. In addition, the interrogation technique will be optimized and applied to numerous other cases where zero current potentiometry has shown, or is expected to show, unsatisfactory sensor behavior.