IMPROVING THE DETECTION LIMIT OF POTENTIOMETRIC SENSORS

提高电位传感器的检测限

• 批准号: 6725983
• 负责人: ERIC BAKKER
• 金额: $ 15.77万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-25 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6725983
• 关键词: biomedical equipment development; biosensor device; electrodes; fluorescent dye /probe; heavy metals; mathematical model; membrane; miniature biomedical equipment

This research establishes the experimental and theoretical foundation for the development of potentiometric ion sensors with detection limits in the micromolar to picomolar range. The flux of analyte ions from the membrane into the sample which ordinarily perturbs the sample locally at the sensing surface will be minimized by chemical means. Relevant zero-current ion fluxes within plasticized polymeric membranes will be monitored with fluorescence imaging techniques and correlated to appropriate potential drift experiments. Detailed ion flux diffusion models will be developed and correlated to experimental findings. Diffusion coefficients within the membrane phase will be systematically altered with new polyurethane materials with immobilized ionophoric components in view of low detection limit sensing applications. Such polymers will be also used to devise multi-layered membrane phases, which will be applied to generate robust ion gradients within the membrane. Miniature, micrometer sized optical sensor based on the same ionophoric membrane components will be optimized for low detection limit applications and their performance critically compared to their potentiometric counterparts.

本研究建立了实验和理论 电位型离子传感器的研制基础 极限在微摩尔至皮摩尔范围内。分析物离子的流量从 膜进入样品，这通常会在样品的表面局部扰动样品。 将通过化学方法使传感表面最小化。相关零流离子 将通过以下方式监测增塑聚合物膜内的通量 荧光成像技术，并与适当的潜在漂移 实验将开发详细的离子通量扩散模型， 与实验结果相关。膜内扩散系数 相将系统地改变与新的聚氨酯材料， 考虑到低检测限传感的固定化离子载体组分 应用.这种聚合物也将用于设计多层膜 相位，其将被应用以在 膜的基于该传感器的微型微米级光学传感器 离子迁移膜组件将被优化以实现低检测限 应用程序和他们的性能相比，他们的电位 同行