Instrumental Control of Ion-Selective Electrodes

离子选择电极的仪器控制

• 批准号: 7116374
• 负责人: ERIC BAKKER
• 金额: $ 14.25万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116374
• 关键词: analytical method; biomedical equipment development; chemical stability; coulometry; electrochemistry; electrodes; heparin; ion transport; ionophores; membrane activity; membrane potentials; method development; polyion; protamines

DESCRIPTION (provided by applicant): This research establishes the instrumental control of ion-selective electrodes as an alternative measurement principle to traditional zero current potentiometry. While ion-selective electrodes are routine tools in clinical laboratories all over the world, numerous new sensing principles that have recently been developed for ISEs make use of ion fluxes, for which classical potentiometry is no longer an adequate transduction method. It is proposed to apply repetitive current and potential pulses to ion-selective membranes that are designed to lack ion-exchanger properties. The resulting sensor responses have the same look and feel as in zero current potentiometry and will be comprehensively compared to each other. As an initial important application of this method, fully reversible polyion sensors for the detection of the anticoagulant heparin and its antidote protamine will be developed. For this first time, this methodology forms the foundation for a truly continuous monitoring technology for these important analytes in whole blood samples during surgery. In addition, the interrogation technique will be optimized and applied to numerous other cases where zero current potentiometry has shown, or is expected to show, unsatisfactory sensor behavior.

描述(申请人提供)：本研究建立了离子选择电极的仪器控制，作为传统零电流电位法的替代测量原理。虽然离子选择电极是世界各地临床实验室的常规工具，但最近为离子选择电极开发的许多新的传感原理都利用了离子通量，传统的电位法已经不再是一种合适的传感方法。建议将重复的电流和电势脉冲应用于设计为缺乏离子交换性能的离子选择膜。由此产生的传感器响应具有与零电流电位法相同的外观和感觉，并将进行全面的相互比较。作为该方法的首次重要应用，将开发用于检测抗凝血剂肝素及其解毒剂鱼精蛋白的全可逆多离子传感器。这是第一次，这种方法为手术期间全血样本中这些重要分析物的真正持续监测技术奠定了基础。此外，询问技术将得到优化，并应用于许多其他情况，即零电流电位法已经显示或预计将显示不令人满意的传感器行为。