Cell motility within a cell mass

细胞团内的细胞运动

• 批准号: 6950976
• 负责人: Gordon L Hager
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6950976
• 关键词: Dictyostelium; cell cell interaction; cell migration; cell motility; green fluorescent proteins; intracellular transport; microscopy; myosins; protein localization; protein transport; time resolved data

The long term objective of our current research is to understand the mechanisms by which cells move within a three-dimensional, multicellular mass. This movement is more complex than migration of an isolated cell on a flat substrate, because cells within a mass must gain traction on each other and also clear paths for themselves within and through the cell mass. We study such "3D cell motion" using time-lapse 3D microscopy which permits us to observe locomotion in living specimens over extended time periods. Current research focuses on the role of the myosin II molecule in cell motility within the multicellular mass of the model organism Dictyostelium. Using a GFP-tagged mysoin II, we have shown that retraction of the cell rear is accompanied by a characteristic redistribution of the myosin at the cell rear. We have also shown that that this redistribution of myosin is abberant when certain myosin mutants are examined, and that rear retraction is also concomitantly affected. These results are the first to demonstrate a specific functional role for myosin II in rear retraction of a cell.

我们当前研究的长期目标是了解细胞在三维多细胞团中移动的机制。这种运动比单个细胞在平坦基质上的迁移更复杂，因为团内的细胞必须获得彼此的牵引力，并且还为它们自己在细胞团内和通过细胞团清理路径。我们使用延时 3D 显微镜研究这种“3D 细胞运动”，这使我们能够在较长时间内观察活体样本的运动。目前的研究重点是肌球蛋白 II 分子在模式生物盘基网柄菌多细胞团内细胞运动中的作用。使用 GFP 标记的肌球蛋白 II，我们发现细胞后部的收缩伴随着细胞后部肌球蛋白的特征性重新分布。我们还表明，当检查某些肌球蛋白突变体时，肌球蛋白的这种重新分布是异常的，并且后部回缩也随之受到影响。这些结果首次证明了肌球蛋白 II 在细胞后缩回中的特定功能作用。