Arrays of Receptors for Infectious Disease Diagnosis

用于传染病诊断的受体阵列

• 批准号: 6744033
• 负责人: Frances S. Ligler
• 金额: $ 60.01万
• 依托单位: U.S. NAVAL RESEARCH LABORATORY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6744033
• 关键词: adhesin; antibiotics; antibody; antigen antibody reaction; bacterial antigens; bacterial toxins; bioengineering /biomedical engineering; biohazard detection; biosensor device; bioterrorism /chemical warfare; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; hemoprotein; membrane proteins; microarray technology; oligosaccharides; siderophores

DESCRIPTION (provided by applicant): Goal: Develop an array of capture molecules for pathogens, intended for use in a rapid screening format, that includes the following: 1. specific capture molecules for different species of microorganisms or their toxic products; 2. semi-selective capture molecules that identify pathogens based on mechanisms involved in the process; of pathogenicity; 3. indicators of potential susceptibility to antibiotics. The first step in this process is to modify candidates for the array and immobilize them in a sensor for rapid characterization. The candidates will include antibodies, oligosaccharides, adhesions, siderophores, combinatorial peptides which bind membrane proteins on the pathogen, and antibiotics. The candidates will be exposed to a broad spectrum of pathogens and toxins to determine both specificity and binding affinity. The elements of the array will be selected to provide the widest amount of information possible: species if possible, mechanism of infection or pathogenicity, and antibiotic susceptibility. The array elements will then be transferred to a corporate partner with demonstrated capability of producing thousands of sensor arrays for clinical use.

描述（由申请人提供）：目标：开发病原体捕获分子阵列，用于快速筛选格式，包括以下内容：针对不同种类的微生物或其有毒产物的特定捕获分子；2. 半选择性捕获分子，根据过程中涉及的机制识别病原体；致病性;3. 抗生素潜在敏感性指标。该过程的第一步是修改候选阵列并将其固定在传感器中以进行快速表征。候选物质包括抗体、低聚糖、黏附物、铁载体、结合病原体膜蛋白的组合肽和抗生素。候选人将暴露于广泛的病原体和毒素，以确定特异性和结合亲和力。将选择阵列的元素以提供尽可能多的信息：如果可能的话，物种，感染或致病性机制以及抗生素敏感性。阵列元件随后将被转移到具有生产数千个用于临床使用的传感器阵列的能力的公司合作伙伴。