Arrays of Receptors for Infectious Disease Diagnosis

用于传染病诊断的受体阵列

• 批准号: 6889244
• 负责人: Frances S. Ligler
• 金额: $ 59.19万
• 依托单位: U.S. NAVAL RESEARCH LABORATORY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6889244
• 关键词: adhesin; antibiotics; antibody; antigen antibody reaction; bacterial antigens; bacterial toxins; bioengineering /biomedical engineering; biohazard detection; biosensor device; bioterrorism /chemical warfare; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; hemoprotein; membrane proteins; microarray technology; oligosaccharides; siderophores

DESCRIPTION (provided by applicant): Goal: Develop an array of capture molecules for pathogens, intended for use in a rapid screening format, that includes the following: 1. specific capture molecules for different species of microorganisms or their toxic products; 2. semi-selective capture molecules that identify pathogens based on mechanisms involved in the process; of pathogenicity; 3. indicators of potential susceptibility to antibiotics. The first step in this process is to modify candidates for the array and immobilize them in a sensor for rapid characterization. The candidates will include antibodies, oligosaccharides, adhesions, siderophores, combinatorial peptides which bind membrane proteins on the pathogen, and antibiotics. The candidates will be exposed to a broad spectrum of pathogens and toxins to determine both specificity and binding affinity. The elements of the array will be selected to provide the widest amount of information possible: species if possible, mechanism of infection or pathogenicity, and antibiotic susceptibility. The array elements will then be transferred to a corporate partner with demonstrated capability of producing thousands of sensor arrays for clinical use.

描述（由申请人提供）：目标：开发用于快速筛查形式的病原体捕获分子阵列，包括以下内容：1.针对不同种类微生物或其有毒产物的特异性捕获分子; 2.半选择性捕获分子，其基于过程中涉及的机制识别病原体;致病性; 3.可能对抗生素敏感的指标。 该过程的第一步是修改阵列的候选物，并将其放入传感器中进行快速表征。候选物将包括抗体、寡糖、粘附物、铁载体、结合病原体上的膜蛋白的组合肽和抗生素。候选物将暴露于广谱病原体和毒素，以确定特异性和结合亲和力。将选择阵列的元素以提供尽可能广泛的信息：物种（如果可能），感染或致病机制以及抗生素敏感性。然后，阵列元件将被转移到一个公司合作伙伴，该合作伙伴已证明有能力生产数千个传感器阵列用于临床。

项目成果

Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins.

• DOI: 10.3390/s7112808
• 发表时间: 2007-11-16
• 期刊: Sensors (Basel, Switzerland)
• 作者: Kulagina NV;Anderson GP;Ligler FS;Shaffer KM;Taitt CR
• 通讯作者: Taitt CR

Simultaneous determination of kinetic parameters for the binding of cholera toxin to immobilized sialic acid and monoclonal antibody using an array biosensor.

使用阵列生物传感器同时测定霍乱毒素与固定化唾液酸和单克隆抗体结合的动力学参数。

• DOI: 10.1016/j.bios.2005.12.007
• 发表时间: 2006
• 期刊: Biosensors & bioelectronics
• 影响因子: 12.6
• 作者: Ngundi,MiriamM;Taitt,ChrisR;Ligler,FrancesS
• 通讯作者: Ligler,FrancesS

Antimicrobial peptide arrays for detection of inactivated biothreat agents.

用于检测灭活生物威胁剂的抗菌肽阵列。

• DOI: 10.1007/978-1-60327-394-7_11
• 发表时间: 2009
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Taitt,ChrisR;North,StellaH;Kulagina,NadezhdaV
• 通讯作者: Kulagina,NadezhdaV

Detection of bacterial toxins with monosaccharide arrays.

• DOI: 10.1016/j.bios.2005.05.001
• 发表时间: 2006-01
• 期刊: Biosensors & bioelectronics
• 影响因子: 12.6
• 作者: Miriam M. Ngundi;C. Taitt;S. McMurry;D. Kahne;F. Ligler