Arrays of Receptors for Infectious Disease Diagnosis

用于传染病诊断的受体阵列

• 批准号: 6588617
• 负责人: Frances S. Ligler
• 金额: $ 60.57万
• 依托单位: U.S. NAVAL RESEARCH LABORATORY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6588617
• 关键词: adhesin; antibiotics; antibody; antigen antibody reaction; bacterial antigens; bacterial toxins; bioengineering /biomedical engineering; biohazard detection; biosensor device; bioterrorism /chemical warfare; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; hemoprotein; membrane proteins; microarray technology; oligosaccharides; siderophores

DESCRIPTION (provided by applicant): Goal: Develop an array of capture molecules for pathogens, intended for use in a rapid screening format, that includes the following: 1. specific capture molecules for different species of microorganisms or their toxic products; 2. semi-selective capture molecules that identify pathogens based on mechanisms involved in the process; of pathogenicity; 3. indicators of potential susceptibility to antibiotics. The first step in this process is to modify candidates for the array and immobilize them in a sensor for rapid characterization. The candidates will include antibodies, oligosaccharides, adhesions, siderophores, combinatorial peptides which bind membrane proteins on the pathogen, and antibiotics. The candidates will be exposed to a broad spectrum of pathogens and toxins to determine both specificity and binding affinity. The elements of the array will be selected to provide the widest amount of information possible: species if possible, mechanism of infection or pathogenicity, and antibiotic susceptibility. The array elements will then be transferred to a corporate partner with demonstrated capability of producing thousands of sensor arrays for clinical use.

描述(申请人提供)：目标：开发一系列病原体捕获分子，用于快速筛查，其中包括：1.针对不同种类微生物或其有毒产品的特定捕获分子；2.根据过程中涉及的机制识别病原体的半选择性捕获分子；致病性；3.抗生素潜在敏感性指标。这一过程的第一步是修改阵列的候选者，并将它们固定在传感器中，以便快速表征。候选药物将包括抗体、低聚糖、粘连、铁载体、结合病原体上膜蛋白的组合肽和抗生素。候选人将接触到广泛的病原体和毒素，以确定特异性和结合亲和力。将选择阵列中的元素以提供尽可能广泛的信息：如果可能的话，物种、感染或致病机制，以及抗生素敏感性。然后，阵列元素将被转移到一个企业合作伙伴手中，该合作伙伴具有生产数千个临床使用的传感器阵列的能力。