TEMPERATURE PROFILE SPECTROSCOPY

温度分布光谱

• 批准号: 6736812
• 负责人: George Eric Plum
• 金额: $ 36.8万
• 依托单位: IBET, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6736812
• 关键词: analytical chemistry; bioengineering /biomedical engineering; biomedical equipment development; biotechnology; calorimetry; computer program /software; fiber optics; high throughput technology; macromolecule; spectrometry; thermodynamics; ultraviolet spectrometry

DESCRIPTION (provided by applicant): Rapid advances in biotechnology have presented unprecedented amounts of new information about the sequences of nucleic acids and proteins. This abundance of information has created an experimental bottleneck. To realize the full potential of the new information, the newly discovered molecules must be characterized in terms of their function, structure, energetics and their interactions. Many of the important determinants of biological function, as well as applications in screening and design of diagnostic and therapeutic agents, are thermodynamic. High-throughput analytical instrumentation is necessary to characterize thermodynamically the vast array of new molecules. Current methods lack the speed and the ability to couple with other devices necessary for high throughput applications. The long-term goal of this research is to address the need for rapid, accurate thermodynamic characterization of biological macromolecules by developing a family of analytical instruments based a new idea called Temperature Profile Spectroscopy. By measurement of optical properties simultaneously over a range of temperature, a reduction of greater than 100 fold in the time required for a thermodynamic characterization of biological macromolecules in solution will be achieved. Development of the Temperature Profile Spectroscopy methodology will permit a significant acceleration in the thermodynamic characterization of biological macromolecules and their interactions, as well as, in the rational design of probe molecules for use in screening and other applications.

描述(申请人提供)：生物技术的快速发展提供了前所未有的大量有关核酸和蛋白质序列的新信息。如此丰富的信息造成了一个实验瓶颈。为了实现新信息的全部潜力，新发现的分子必须从它们的功能、结构、能量学和它们的相互作用方面进行表征。生物功能的许多重要决定因素，以及在诊断和治疗药物的筛选和设计中的应用，都是热力学的。高通量的分析仪器对于从热力学上表征大量新分子是必要的。目前的方法缺乏与高吞吐量应用所需的其他设备耦合的速度和能力。这项研究的长期目标是通过开发一系列基于称为温度分布光谱的新概念的分析仪器来满足对生物大分子快速、准确的热力学表征的需求。通过在一定温度范围内同时测量光学性质，生物大分子在溶液中的热力学表征所需的时间将减少100倍以上。温度分布光谱方法的发展将大大加快生物大分子及其相互作用的热力学表征，以及用于筛选和其他应用的探针分子的合理设计。