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Rapid Screening of Nucleic Acid Energetics

核酸能量学的快速筛选

基本信息

批准号：
7943143
负责人：
George Eric Plum
金额：
$ 34.16万
依托单位：
IBET, INC.
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-20 至 2013-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7943143
关键词：
Academia Affinity Base Sequence Behavior Binding Biochemistry Biological Biophysics Biotechnology Cells Characteristics Chemistry Collection Complex Computer software DNA Data Data Analyses Development Devices Diagnostic Disease Dissociation Documentation Drug Stability Dyes Equilibrium Exposure to Fluorescent Dyes Heating High temperature of physical object Human Genome Industry Knowledge Laboratories Manuals Manufacturer Name Measurement Measures Methods Metric Modeling Monitor Nucleic Acid Hybridization Nucleic Acids Optics Performance Phase Positioning Attribute Preparation Price Production Proteins Protocols documentation Reader Resolution Salts Sampling Screening procedure Series Solutions Solvents Specificity Spectrum Analysis Speed System Techniques Temperature Testing Therapeutic Thermodynamics Time base biophysical chemistry chromophore commercialization design high throughput screening improved instrument instrumentation macromolecule meetings melting meter novel nucleic acid analog nucleic acid stability nucleic acid structure prototype research study small molecule

项目摘要

DESCRIPTION (provided by applicant): Recent advances, perhaps most visible in the Human Genome Initiative, have presented new and compelling means and objectives to exploit the exquisite specificity of nucleic acid hybridization. Precise knowledge of the temperature dependent stability of nucleic acid (and nucleic acid analog) complexes is necessary for the rational design and optimization of hybridization probes for use in high throughput screening, nucleic acid based diagnostics and therapeutics, and numerous emerging biotechnology applications. The primary objective of the proposed project is to develop a high-throughput means of obtaining accurate thermal and thermodynamic data for nucleic acid complexes by measurement and analysis of optically-monitored, temperature-induced, order-disorder transitions. The proposed device will operate analogously to a pH meter, the operator will load a nucleic acid complex sample and after a short settling time a numerical result will be displayed. The device will provide, in a matter of seconds, a complete thermodynamic analysis capable of predicting accurately the stability of the nucleic acid complex at any temperature. PUBLIC HEALTH RELEVANCE: The primary objective of the proposed project is to develop a high-throughput means of obtaining accurate thermal and thermodynamic data for nucleic acid complexes by measurement and analysis of optically- monitored, temperature-induced, order-disorder transitions. This information is useful in designing and troubleshooting probes and in more fundamental studies of nucleic acids.

描述（由申请人提供）：最近的进展，也许在人类基因组计划中最为明显，已经提出了新的、令人信服的手段和目标，以利用核酸杂交的精致特异性。准确了解核酸（和核酸类似物）复合物的温度依赖性稳定性对于合理设计和优化用于高通量筛选、基于核酸的诊断和治疗以及众多新兴生物技术应用的杂交探针是必要的。该项目的主要目标是开发一种高通量方法，通过测量和分析光学监测、温度诱导的有序-无序转变来获得核酸复合物的准确热和热力学数据。所提出的设备将类似于 pH 计进行操作，操作员将加载核酸复合物样品，并在短暂的沉降时间后显示数字结果。该设备将在几秒钟内提供完整的热力学分析，能够准确预测核酸复合物在任何温度下的稳定性。公共健康相关性：拟议项目的主要目标是开发一种高通量方法，通过测量和分析光学监测、温度诱导的有序-无序转变来获得核酸复合物的准确热和热力学数据。这些信息对于探针的设计和故障排除以及更基础的核酸研究非常有用。