TEMPERATURE PROFILE SPECTROSCOPY

温度分布光谱

• 批准号: 6855093
• 负责人: George Eric Plum
• 金额: $ 37.35万
• 依托单位: IBET, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6855093
• 关键词: analytical chemistry; bioengineering /biomedical engineering; biomedical equipment development; biotechnology; calorimetry; computer program /software; fiber optics; high throughput technology; macromolecule; spectrometry; thermodynamics; ultraviolet spectrometry

DESCRIPTION (provided by applicant): Rapid advances in biotechnology have presented unprecedented amounts of new information about the sequences of nucleic acids and proteins. This abundance of information has created an experimental bottleneck. To realize the full potential of the new information, the newly discovered molecules must be characterized in terms of their function, structure, energetics and their interactions. Many of the important determinants of biological function, as well as applications in screening and design of diagnostic and therapeutic agents, are thermodynamic. High-throughput analytical instrumentation is necessary to characterize thermodynamically the vast array of new molecules. Current methods lack the speed and the ability to couple with other devices necessary for high throughput applications. The long-term goal of this research is to address the need for rapid, accurate thermodynamic characterization of biological macromolecules by developing a family of analytical instruments based a new idea called Temperature Profile Spectroscopy. By measurement of optical properties simultaneously over a range of temperature, a reduction of greater than 100 fold in the time required for a thermodynamic characterization of biological macromolecules in solution will be achieved. Development of the Temperature Profile Spectroscopy methodology will permit a significant acceleration in the thermodynamic characterization of biological macromolecules and their interactions, as well as, in the rational design of probe molecules for use in screening and other applications.

描述（由申请人提供）：生物技术的快速发展已经提供了关于核酸和蛋白质序列的前所未有的大量新信息。如此丰富的信息造成了实验瓶颈。为了实现新信息的全部潜力，必须根据其功能，结构，能量学及其相互作用来表征新发现的分子。许多生物学功能的重要决定因素以及在诊断和治疗剂的筛选和设计中的应用都是热力学的。高通量分析仪器是必要的，以表征大量的新分子。目前的方法缺乏与高通量应用所需的其他设备耦合的速度和能力。这项研究的长期目标是通过开发一系列基于称为温度剖面光谱学的新思想的分析仪器来解决对生物大分子快速，准确热力学表征的需求。通过在一个温度范围内同时测量光学性质，将实现溶液中生物大分子的热力学表征所需时间的大于100倍的减少。温度分布光谱学方法的发展将使生物大分子及其相互作用的热力学表征以及用于筛选和其他应用的探针分子的合理设计得到显着加速。