Dry-reagent methods for detection of iodine in urine

描述（由申请人提供）：本项目的长期目标是开发快速、准确和可靠的尿碘测定方法和试剂盒。方法和检测试剂盒必须便宜且易于使用，以便在现场条件下进行碘的测定。将开发用于检测尿中碘的干试剂测试条方法。试纸条法将通过快速“浸渍和读取”程序检测碘。当试纸浸入被测样品时，指示垫的颜色将根据碘水平发生变化。除了指示板中包含的化学品外，不需要单独的化学品供应。无需耗时的试剂工作，如称重、混合、体积测量等. 今天，全世界约有16亿人仍然生活在缺碘症并发症的威胁之下，这些并发症包括：先天性粘液水肿（克汀病）、脑损伤、甲状腺功能减退和甲状腺肿。由于碘缺乏症可以通过补充碘盐很容易地治愈，因此对缺碘地区的人口进行筛查对于有效规划、执行和改进缺碘症预防措施至关重要。筛查人群碘缺乏和摄入的最佳方法是测量尿碘。筛查对全世界所有贫穷、偏远和服务不足的社区都非常重要;在发达国家也很重要。为了控制碘缺乏病，需要开发新的、快速、准确、可靠和廉价的尿碘测量测试试剂盒，以取代目前使用的基于实验室的方法。公共卫生相关性：本项目的主要目的是开发一种快速、廉价、易于使用的干试剂法测定尿中碘。尿中碘的测定对于确定和监测全世界的碘补充计划是必要的。严重的碘缺乏症在美国是罕见的，然而，包括育龄妇女在内的亚人群有轻度至中度碘缺乏症的风险。

DESCRIPTION (provided by applicant): The long term goal of this project is to develop rapid, accurate and reliable methods and test kits for iodine determination in urine. The methods and test kits must be inexpensive and easy-to-use to perform determinations of iodine under field conditions. Dry-reagent test strip methods for detection of iodine in urine will be developed. The test strip method will detect iodine by a fast "dip and read" procedure. When the test strips are dipped in the tested sample, the color of the indicator pad will change in accordance with iodine levels. No separate chemical supply will be needed except for that included in the indicator pad. No time- consuming work with reagents such as weighing, mixing, volume measurement, etc. will be required. Today, about 1.6 billion people worldwide still live under the threat of IDD complications that include: congenital myxedema (cretinism), brain damage, hypothyroidism and goiter. Because iodine deficiency could be readily cured by iodized salt supplementation, screening of the population in iodine-deficient regions is critical for effective planning, implementation and improvement of IDD-preventive measures. The best way to screen for iodine deficiency and intake in populations is to measure iodine in urine. The screening is highly important for all poor, remote and underserved communities worldwide; it is also important in the developed world. In order to control IDD new, rapid, accurate, reliable and inexpensive iodine measurement test kits for urine need to be developed to replace currently used laboratory based methods. PUBLIC HEALTH RELEVANCE: The primary objective of the proposed project is to develop a rapid, inexpensive, easy to use, dry-reagent method for determination of iodine in urine. Determination of iodine in urine is necessary to target and monitor iodine supplementation programs worldwide. Severe iodine deficiency is rare in the United States, however, subpopulations including women of reproductive age are at risk of mild to moderate iodine deficiency.