A system for automated behavioral phenotyping of mice

小鼠自动行为表型分析系统

• 批准号: 6735871
• 负责人: SHANNON R CAMPBELL
• 金额: $ 7万
• 依托单位: KUZER COMPANY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6735871
• 关键词: behavioral /social science research tag; body movement; computer simulation; digital imaging; ethology; laboratory mouse; learning; mathematical model; orientation; phenotype; psychobiology; social behavior; startle reaction; technology /technique development; video recording system; vocalization

DESCRIPTION (provided by applicant): Mice are widely used in genetic and medical research. Because genetic alterations and exposure to drugs can induce subtle behavioral changes, there is a need to automatically recognize and quantify a variety of murine behaviors. We propose a system for automated behavioral phenotyping of mice. The system will acquire digital video data of single or multiple mice on typical bedding material, under day and night lighting conditions. The system will find the mouse contour, the location and orientation of its head, and the tail position. With this information, we can categorize a variety of motions, including locomotion, freezing, grooming, rearing, seizures, stereotypies, and a variety of social behaviors including nosing, ano-genital sniffing, aggression, and mating behaviors. A microphone will be used for recording vocalizations and a speaker will be used for startle tests. Currently, companies screen thousands of mutant mice using devices that are not automated. Thus phenotyping is time consuming, labor intensive, and limited to easily measured physical quantities and common behaviors. Our system will be a commercially viable tool useful to drug companies and scientists, will aid discovery in mouse social behavior, and will help researchers understand how genetic or pharmacological manipulations cause changes in behavior.

描述（申请人提供）：小鼠广泛用于遗传和医学研究。由于遗传改变和暴露于药物会引起微妙的行为变化，因此需要自动识别和量化各种小鼠行为。我们提出了一个系统的自动行为表型小鼠。该系统将在白天和夜间照明条件下采集单个或多个小鼠在典型垫料上的数字视频数据。系统将找到鼠标的轮廓、头部的位置和方向以及尾部的位置。有了这些信息，我们可以对各种动作进行分类，包括运动、冻结、梳理、饲养、癫痫发作、刻板印象，以及各种社会行为，包括嗅闻、肛门生殖器嗅闻、攻击和交配行为。麦克风将用于记录发声，扬声器将用于惊吓测试。 目前，公司使用非自动化设备筛选数千只突变小鼠。因此，表型分析是耗时的、劳动密集型的，并且限于容易测量的物理量和常见行为。我们的系统将是一个商业上可行的工具，对制药公司和科学家有用，将有助于发现小鼠的社会行为，并将帮助研究人员了解遗传或药理学操作如何引起行为的变化。