Evaluation of Laboratory Diagnostic Methods

实验室诊断方法评价

• 批准号: 6825594
• 负责人: GLEN HORTIN
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6825594
• 关键词: blood chemistry; blood disorder diagnosis; blood glucose; blood protein disorder; cardiovascular disorder diagnosis; diabetes mellitus; diagnosis design /evaluation; diagnosis quality /standard; heart disorder; human tissue; kidney disorder; kidney disorder diagnosis; mass spectrometry; matrix assisted laser desorption ionization; metabolism disorder diagnosis; urinalysis

Goals of this project are to improve clinical laboratory methods for diagnosis of disease. Studies include analysis of clinical laboratory practices, analysis of the accuracy of laboratory tests, and development of new tests and testing technologies. The data from a national survey of laboratory practices for measurement of parathyroid hormone during surgery on parathyroid glands was evaluated and published. Data was evaluated from a multicenter evaluation of the performance of glucose meters used by diabetics to measure their blood sugar levels. The study provides one of the most extensive evaluations of the consistency and accuracy of blood glucose meters. This will be useful for establishing goals for the performance of glucose meters and suggests approaches for improving their accuracy. Results are in press. A variety of new approaches have been developed for diagnosis of kidney disease. These recent developments were reviewed in a published editorial and ongoing research is seeking to improve the measurement of creatinine and to improve the diagnosis of kidney by laboratory methods. Mass spectrometry methods are under evaluation as a new approach for analysis of blood and urine specimens. Matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry serves as a method for analysis of complex patterns of proteins and peptides in biological fluids. Results of our studies suggest that this method will be useful for study of a number of the small protein components of high-density and low-density lipoproteins (HDL and LDL). These studies may provide new insights into the structure of HDL and LDL that are commonly measured in the laboratory to assess risk for cardiovascular disease. These studies may also identify new technologies that can be applied for routine diagnostic methods. Ongoing studies are examining the interactions of the amino acid homocysteine with plasma proteins in extension of previous published studies that have examined relationships between homocysteine, cysteine, and plasma proteins. Increased concentrations of homocysteine are related to heart disease and abnormal formation of blood clots so there is a need for better understanding of interactions of this amino acid. Other studies have been directed at improving the measurement of the activity of proteases that are involved in many important processes such as blood coagulation. New reagents have been developed for measuring protease activity.

该项目的目标是改善临床实验室诊断疾病的方法。研究包括临床实验室实践的分析，实验室测试的准确性分析，以及新的测试和测试技术的开发。对甲状旁腺手术期间甲状旁腺激素测量实验室实践的全国调查数据进行了评估并发表。数据来自糖尿病患者使用血糖仪测量血糖水平的多中心评价。该研究提供了对血糖仪的一致性和准确性的最广泛的评估之一。这将有助于为血糖仪的性能建立目标，并建议提高其准确性的方法。结果即将公布。已经开发了各种新的方法来诊断肾脏疾病。这些最新进展在已发表的社论中进行了综述，正在进行的研究正在寻求改善肌酐的测量，并通过实验室方法改善肾脏的诊断。质谱法作为血液和尿液样本分析的新方法正在接受评估。基质辅助激光解吸/电离飞行时间（MALDI TOF）质谱法是一种分析生物体液中蛋白质和多肽复杂模式的方法。我们的研究结果表明，这种方法将是有用的高密度和低密度脂蛋白（HDL和LDL）的小蛋白组分的研究。这些研究可能为HDL和LDL的结构提供新的见解，这些结构通常在实验室中测量以评估心血管疾病的风险。这些研究还可以确定可应用于常规诊断方法的新技术。正在进行的研究正在检查氨基酸同型半胱氨酸与血浆蛋白的相互作用，以扩展先前已发表的研究，这些研究检查了同型半胱氨酸，半胱氨酸和血浆蛋白之间的关系。同型半胱氨酸浓度的增加与心脏病和血凝块的异常形成有关，因此需要更好地了解这种氨基酸的相互作用。其他研究已经针对改善蛋白酶活性的测量，所述蛋白酶参与许多重要过程，例如血液凝固。已经开发了用于测量蛋白酶活性的新试剂。