Evaluation of Laboratory Diagnostic Methods

实验室诊断方法评价

• 批准号: 7593113
• 负责人: GLEN HORTIN
• 金额: $ 5.5万
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593113
• 关键词: Affect; Albumins; Area; Binding; Biological Assay; Blood; Cardiovascular Diseases; Cardiovascular system; Clinical; Collection; Complex; Detection; Development; Diagnosis; Diagnostic; Diagnostic Procedure; Disease; Evaluation; Goals; Homocysteine; Homocystine; Injury; Kidney; Laboratories; Measurement; Measures; Metabolic Diseases; Methods; Modification; Nonesterified Fatty Acids; Plasma; Plasma Proteins; Procedures; Proteins; Risk Factors; Specimen; Technology; Testing; Thrombosis; Tube; Urine; Work; clinical application; disulfide bond; evaluation/testing; improved; interest; plasma protein fraction; sample collection; urinary

Goals of this project are to improve clinical laboratory methods for diagnosis of disease. Studies include analysis of clinical laboratory practices, analysis of the accuracy of laboratory tests, and development of new tests and testing technologies. The major efforts over the past year have been to continue work on the effect of blood collection tubes on assays, to characterize urinary proteins of potential diagnostic significance, and to evaluate the distribution of homocysteine between different plasma protein fractions. 1) Analyses of effects of blood collection tubes found complex interactions with the analysis of free fatty acids probably due to modification of the specimen rather than direct interaction with the assay. These efforts help identify optimal collection procedures and tubes for collection of specimens for free fatty acid analysis and point to new mechanisms by which laboratory results may be affected by collection tubes. 2) Studies of urinary proteins have focused mainly on analysis of urinary albumin which serves as one of the most widely used markers for early glomerular injury. Our laboratory has examined how clinical assays measure modified forms of albumin that may occur in urine and have undertaken efforts to characterize the modified forms of albumin in urine. These efforts are expected to contribute to better clinical application and interpretation of urinary albumin measurements for the detection of kidney injury. 3) Plasma homocysteine has been of great interest as a potential risk factor for cardiovascular disease and thrombosis. We have examined the linkage of homocysteine to plasma proteins via disulfide bonds. Our analyses may subdivide the total homocysteine content into different subfractions bound to different proteins, and we have examined the differential exchange of homocysteine bound to different protein fractions. These studies seek to understand the interactions of homocysteine with plasma proteins and to identify subfractions of homocysteine that may serve as better indicators of cardiovascular disease.

该项目的目标是改进诊断疾病的临床实验室方法。研究包括分析临床实验室实践，分析实验室测试的准确性，以及开发新的测试和测试技术。在过去的一年里，主要的努力是继续研究血液采集管对分析的影响，表征具有潜在诊断意义的尿蛋白，并评估同型半胱氨酸在不同血浆蛋白组分之间的分布。1)对血液采集管的影响分析发现，与游离脂肪酸分析的复杂相互作用可能是由于样品的修改，而不是与分析的直接相互作用。这些努力有助于确定收集用于游离脂肪酸分析的标本的最佳收集程序和管子，并指出了采集管可能影响实验室结果的新机制。2)尿蛋白的研究主要集中在尿白蛋白的分析上，尿白蛋白是早期肾小球损伤最广泛使用的标记物之一。我们的实验室研究了临床检测如何测量尿液中可能出现的修饰形式的白蛋白，并努力确定尿液中修饰形式的白蛋白。预计这些努力将有助于更好地临床应用和解释尿白蛋白测量以检测肾脏损伤。3)血浆同型半胱氨酸是心血管疾病和血栓形成的潜在危险因素。我们研究了同型半胱氨酸通过二硫键与血浆蛋白的连接。我们的分析可以将总同型半胱氨酸含量细分为与不同蛋白质结合的不同亚组分，我们还研究了同型半胱氨酸与不同蛋白质组份结合的差异交换。这些研究试图了解同型半胱氨酸与血浆蛋白的相互作用，并确定同型半胱氨酸的亚组分可能作为心血管疾病的更好指标。