Macromolecular Substrates For Enzymes

酶的大分子底物

• 批准号: 6546547
• 负责人: GLEN HORTIN
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6546547
• 关键词: allosteric site; chemical structure; chemical synthesis; clinical research; crosslink; diagnosis design /evaluation; diagnosis quality /standard; disease /disorder etiology; enzyme activity; enzyme substrate; human tissue; macromolecule; polymers; thrombin

Measurements of enzyme activity serve as important tools for the diagnosis of disease processes and for basic research. We are seeking to develop new substrates for enzymes that improve the ability to measure or the specificity of measuring enzyme activity. We have found that linking small chromogenic and fluorogenic substrates to polymer molecules serves as an approach to prepare substrates with large molecular size. Initial results are described in Clinical Chemistry 2001;47:215-222. The molecular size of the substrates is determined primarily by the size of the attached polymer so that it is adjustable. This permits substrate size to be analyzed as an independent variable, and it serves as a means of assessing the steric hindrance of the active sites of enzymes. The large synthetic substrates serve as better models of natural substrates that are of large molecular size such as the protein substrates of physiological proteinases and the large starch substrates of amylase. Use of large substrates appears to distinguish free protease molecules from those complexed with the inhibitor alpha-2-macroglobulin. Thus, these substrates appear to have utility for the more accurate measurement of the functional activity of plasma proteinases such as thrombin.

酶活性的测量是诊断疾病过程和基础研究的重要工具。我们正在寻求开发新的酶底物，以提高测量酶活性的能力或测量酶活性的特异性。我们已经发现，连接小的显色和荧光底物的聚合物分子作为一种方法，以制备大分子尺寸的基板。初始结果描述于Clinical Chemistry 2001;47：215-222中。基质的分子大小主要由附着的聚合物的大小决定，因此是可调节的。这允许底物大小作为独立变量进行分析，并且它用作评估酶活性位点的空间位阻的手段。大的合成底物充当大分子尺寸的天然底物的更好的模型，例如生理蛋白酶的蛋白质底物和淀粉酶的大淀粉底物。使用大底物似乎可以区分游离蛋白酶分子和与抑制剂α-2-巨球蛋白复合的蛋白酶分子。因此，这些底物似乎可用于更准确地测量血浆蛋白酶如凝血酶的功能活性。