Experimental Hematopoietic Stem Cell Growth

实验性造血干细胞生长

• 批准号: 6685961
• 负责人: SAUL Joseph SHARKIS
• 金额: $ 50.7万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6685961
• 关键词: apoptosis; cell cell interaction; cell cycle; cell differentiation; cell growth regulation; cell population study; cell proliferation; cell sorting; cell type; cyclin dependent kinase; embryonic stem cell; enzyme inhibitors; gene expression; hematopoiesis; hematopoietic stem cells; laboratory mouse; phenotype; tissue /cell preparation

DESCRIPTION (provided by applicant): Hematopoietic stem cells (HSC) have been the subject of intense investigation for many years. Until recently, the studies primarily examined populations of cells containing very few actual HSC. The rarity of these cells has made it difficult to obtain sufficient numbers of cells for study. Compounding this problem is the potential functional phenotypic diversity of the HSC populations. We have developed an HSC tracking assay which has allowed us to recover HSC following bone marrow transplantation into murine hosts which will allow us for the first time to study both the molecular and cellular characteristics of a potentially homogeneous population of HSC from adult bone marrow. The functional aspects include stem cell proliferation, differentiation, survival and homing in-vitro and in-vivo. The phenotypic characterization includes novel (as well as those suggested from population studies) cell surface markers as well as molecular regulatory molecules such as p27, a cyclin kinase inhibitor which could be responsible for HSC quiescence. In addition, accessory cell populations may very well play a regulatory role in HSC functions. It is our plan to be able to distinguish between HSC cellular divisions which result in maintenance of the HSC compartment, expansion of the compartment or depletion of the compartment. Finally, we will continue to examine developmentally immature HSC for engraftment potential gene expression and plasticity.

描述（由申请人提供）：造血干细胞（HSC）已被 多年来一直在进行深入调查直至最近缅甸国内 研究主要检查含有非常少的实际HSC的细胞群。 这些细胞的稀有性使得难以获得足够数量的 细胞研究。使这个问题复杂化的是潜在的泛函 HSC群体的表型多样性。我们开发了HSC跟踪 这一试验使我们能够在骨髓移植后恢复HSC 这将使我们能够第一次研究 潜在同质群体的分子和细胞特征 从成人骨髓中提取HSC。功能方面包括干细胞 体外和体内的增殖、分化、存活和归巢。的 表型表征包括新的（以及由 细胞表面标志物以及分子调控 分子如p27，一种细胞周期蛋白激酶抑制剂， HSC静止。此外，辅助细胞群可能很好地发挥作用， HSC功能的调节作用。我们的计划是能够区分 在HSC细胞分裂之间， 在一些实施例中，所述腔室的腔室容积可以是腔室容积的增加、腔室容积的增加或腔室容积的减少。 最后，我们将继续研究发育不成熟的HSC， 移植潜力基因表达和可塑性。