Cerebellar Circuitry and Plasticity in an Electric Fish

电鱼的小脑回路和可塑性

• 批准号: 6837654
• 负责人: VICTOR Z. HAN
• 金额: $ 19.88万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6837654
• 关键词: Cerebellar; Circuitry; Plasticity; Electric; Fish

EXCEED THE SPACE PROVIDED. The neuronal circuitryof the cerebellum appears to be relativelysimple and well defined, but the computation performed by this circuitry is not understood. In addition, forms of synaptic plasticityhave been defined in the cerebellum that may be responsiblefor motor learning, but criticalquestions regarding this plasticityare still unanswered. The objective of the proposed research is to address fundamental issues cerebellar circuitry and plasticitythrough a study of the unique cerebellum of mormyridelectricfish. Two features of the mormyrid cerebellum make itwell suitedto address these issues: 1) Purkinjecells and cerebellar output cells (cells of the cerebellar nuclei in mammals) are adjacent to each other in mormyridfish, making possibleexperiments on integrationand plasticityat the important synapse between these two cell types that can not be done in mammals. 2) The regionsof terminationfor climbingfiber and parallel fiber inputs on Purkinjecellsare distinctand well separated in the mormyrid but not inthe mammal. This separation facilitates the determination of intrinsicdifferences between the two dendritic regions and the determination of how the two inputs interactto generate synaptic plasticity. The project has two Specific Aims: 1) to determine the basic synaptic and intrinsicphysiologyof Purkinjeand cerebellar output cells in mormyrid electricfish; and 2) to determine the types of plasticityat parallel fiber and Purkinje cellsynapses in mormyrid electricfish. The primary method will be whole-cell patch recordingin in vitro slices, combined with labelingof recorded elements for morphologicalidentification. PERFORMANCE SITE ========================================Section End===========================================

超出提供的空间。小脑的神经元回路似乎相对简单和明确，但这个回路进行的计算并不为人所知。此外，小脑中可能负责运动学习的突触可塑性的形式已经被定义，但关于这种可塑性的关键问题仍然没有答案。这项拟议研究的目的是通过对桑鱼独特的小脑的研究，解决小脑回路和可塑性的基本问题。小脑的两个特点使得它非常适合于解决这些问题：1)在桑鱼中，浦肯氏细胞和小脑输出细胞(哺乳动物的小脑核细胞)彼此相邻，这使得在这两种细胞类型之间的重要突触上进行整合和可塑性的实验成为可能，而这在哺乳动物中是不可能完成的。2)爬行纤维和平行纤维传入的终止区在胚胎期有明显的分离，但在哺乳动物中没有。这种分离有助于确定两个树突区域之间的内在差异，并确定两个输入如何相互作用以产生突触可塑性。该项目有两个具体目标：1)确定桑鱼浦肯野和小脑输出细胞的基本突触和内在生理学；2)确定桑鱼平行纤维和浦肯野细胞突触的可塑性类型。主要的方法将是在体外切片中进行全细胞斑块记录，并结合所记录的元素的标记进行形态鉴定。表演网站========================================Section End===========================================