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Cerebellar Circuitry and Plasticity in an Electric Fish

电鱼的小脑回路和可塑性

基本信息

批准号：
7162610
负责人：
VICTOR Z. HAN
金额：
$ 18.85万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-01-01 至 2008-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7162610
关键词：
Address Affect Attention Axon Calcium Calcium Spikes Cell Communication Cell physiology Cells Cerebellar Nuclei Cerebellar cortex structure Cerebellum Characteristics Chemosensitization Chromosome Pairing Collaborations Condition Dendrites Dependence Distal Efferent Neurons Electric Fish Electrodes Elements Excitatory Postsynaptic Potentials Fiber Figs - dietary Fishes Foundations Frequencies Future Image In Vitro Injection of therapeutic agent Label Long-Term Depression Mammals Mental Depression Methods Mormyridae Motor Netherlands Neurons Nuclear Output Personal Satisfaction Physiologic pulse Physiology Plastics Principal Investigator Procedures Property Protocols documentation Pulse taking Purkinje Cells Range Recurrence Relative (related person)Research Research Personnel Sensory Process Site Slice Sodium Spatial Distribution Stimulus Structure Structure of molecular layer of cerebellar cortex Structure of purkinje fibers Synapses Synaptic plasticity System Testing Time Universities base biocytin cell cortex cell type in vivo insight motor learning postsynaptic presynaptic programs research study response size synaptic depression tool

项目摘要

The neuronal circuitryof the cerebellum appears to be relativelysimple and well defined, but the computation performed by this circuitry is not understood. In addition, forms of synaptic plasticityhave been defined in the cerebellum that may be responsiblefor motor learning, but criticalquestions regarding this plasticityare still unanswered. The objective of the proposed research is to address fundamental issues cerebellar circuitry and plasticitythrough a study of the unique cerebellum of mormyridelectricfish. Two features of the mormyrid cerebellum make itwell suitedto address these issues: 1) Purkinjecells and cerebellar output cells (cells of the cerebellar nuclei in mammals) are adjacent to each other in mormyridfish, making possibleexperiments on integrationand plasticityat the important synapse between these two cell types that can not be done in mammals. 2) The regionsof terminationfor climbingfiber and parallel fiber inputs on Purkinjecellsare distinctand well separated in the mormyrid but not inthe mammal. This separation facilitates the determination of intrinsicdifferences between the two dendritic regions and the determination of how the two inputs interactto generate synaptic plasticity. The project has two Specific Aims: 1) to determine the basic synaptic and intrinsicphysiologyof Purkinjeand cerebellar output cells in mormyrid electricfish; and 2) to determine the types of plasticityat parallel fiber and Purkinje cellsynapses in mormyrid electricfish. The primary method will be whole-cell patch recordingin in vitro slices, combined with labelingof recorded elements for morphologicalidentification.

小脑的神经元回路似乎相对简单且明确，但不理解由该电路执行的计算。此外，突触的形式可塑性在小脑中被定义为可能负责运动学习，关于这种可塑性的关键问题仍然没有答案。建议的目标研究的目的是通过对小脑的研究来解决小脑回路和可塑性的基本问题。长鳍电鱼独特的小脑。莫米里德小脑的两个特征使它很好地适用于解决这些问题：1）浦肯野细胞和小脑输出细胞（小脑的细胞）哺乳动物中的细胞核）在墨鱼中彼此相邻，这使得实验成为可能在这两种细胞类型之间的重要突触处的整合和可塑性，在哺乳动物中完成。2)上的爬纤和平行纤输入的终止区域浦肯氏细胞在鱼科动物中是独特的，分离得很好，但在哺乳动物中却不是。这分离有助于确定两个树突区域之间的本质差异以及确定两个输入如何相互作用以产生突触可塑性。该项目有两个具体目标：1）确定基本的突触和内在生理学，浦肯野氏细胞和小脑输出细胞; 2）确定长鳍电鱼平行纤维和浦肯野细胞突触的可塑性。的主要方法将在体外切片中进行全细胞贴片记录，并结合记录元素的标记，形态学鉴定