CIRCUITRY AND PLASTICITY IN THE CEREBELLUM OF AN ELECTRIC FISH

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 关于哺乳动物的小脑，人们知道的很多，但对它的局部电路执行的计算以及它在信息处理中的作用仍然缺乏清楚的了解。这个项目的目的是通过对电鱼独特的小脑的研究来洞察小脑的一般功能。该项目的目标是建立桑丘小脑功能回路、突触可塑性和信息处理的主要特征。主要的方法是细胞内记录，染色从体外切片制备和体内整体动物制备中用于形态鉴定的记录元件，辅之以药理学方法和免疫细胞化学。该项目的具体目标是：(1)确定桑麦小脑的基本功能回路。(2)确定平行纤维突触和浦肯野细胞突触不同类型的可塑性。(3)通过研究电感觉刺激的影响、EOD运动指令信号的影响以及这两种信号之间的相互作用来描述瓣膜的信息加工。在特定目标1和2下的体外切片制备中获得的结果将与在特定目标3下在完整动物中获得的结果相结合，以了解瓣膜中正常感觉和运动信息的处理以及这种处理是如何通过经验改变的。每个水平的发现都将与其他实验室在哺乳动物系统中的发现进行比较，以确定Mormyid研究在哪里可以为哺乳动物的小脑功能提供有用的视角或见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much is known about the mammalian cerebellum, but a clear understanding of the computation performed by its local circuitry and of its role in information processing are still lacking. This project is directed at obtaining insight into cerebellar function in general through a study of the unique cerebellum of mormyrid electric fish. The goals of the project are to establish the major features of functional circuitry, synaptic plasticity and information processing in the mormyrid cerebellum. The primary method will be intracellular recording with staining of recorded elements for morphological identification from an in vitro slice preparation and in vivo whole animal preparation, with pharmacological methods supplemented with immunocytochemistry. The Specific Aims of the project are: (1) To determine the basic functional circuit of the mormyrid cerebellum. (2) To determine the different types of plasticity at parallel fiber and Purkinje cell synapses. (3) To describe information processing in the valvula by examining the effects of electrosensory stimuli, the effects of EOD motor command signals and the interaction between these two types of signals. The findings obtained in the in vitro slice preparation under Specific Aims 1 and 2 will be integrated with the findings obtained in the intact animal under Specific Aim 3 to understand the processing of normal sensory and motor information in the valvula and how that processing is altered by experience. Findings at each level will be compared with the findings in mammalian systems that are obtained in other laboratories to see where the mormyrid studies can provide useful perspectives or insights into mammalian cerebellar function.