CIRCUITRY AND PLASTICITY IN THE CEREBELLUM OF AN ELECTRIC FISH

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们对哺乳动物小脑的了解很多，但对它的局部电路进行的计算及其在信息处理中的作用仍然缺乏清晰的了解。本计画旨在借由对长颌电鱼独特的小脑的研究，来了解小脑的一般功能。本研究的目的是建立拟鳚小脑的功能回路、突触可塑性和信息处理的主要特征。主要方法是细胞内记录，对记录的元素进行染色，以从体外切片制备物和体内整个动物制备物中进行形态学鉴定，并辅以免疫细胞化学的药理学方法。本课题的具体目的是：（1）确定海马小脑的基本功能回路。(2)确定平行纤维和浦肯野细胞突触的不同类型的可塑性。(3)通过检查电感觉刺激的作用、EOD运动命令信号的作用以及这两种类型信号之间的相互作用来描述瓣膜中的信息处理。将特定目标1和2下体外切片制备中获得的结果与特定目标3下完整动物中获得的结果相结合，以了解瓣膜中正常感觉和运动信息的处理以及经验如何改变该处理。每个层次的研究结果将与在其他实验室获得的哺乳动物系统中的研究结果进行比较，以了解mormyrid研究可以为哺乳动物小脑功能提供有用的观点或见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much is known about the mammalian cerebellum, but a clear understanding of the computation performed by its local circuitry and of its role in information processing are still lacking. This project is directed at obtaining insight into cerebellar function in general through a study of the unique cerebellum of mormyrid electric fish. The goals of the project are to establish the major features of functional circuitry, synaptic plasticity and information processing in the mormyrid cerebellum. The primary method will be intracellular recording with staining of recorded elements for morphological identification from an in vitro slice preparation and in vivo whole animal preparation, with pharmacological methods supplemented with immunocytochemistry. The Specific Aims of the project are: (1) To determine the basic functional circuit of the mormyrid cerebellum. (2) To determine the different types of plasticity at parallel fiber and Purkinje cell synapses. (3) To describe information processing in the valvula by examining the effects of electrosensory stimuli, the effects of EOD motor command signals and the interaction between these two types of signals. The findings obtained in the in vitro slice preparation under Specific Aims 1 and 2 will be integrated with the findings obtained in the intact animal under Specific Aim 3 to understand the processing of normal sensory and motor information in the valvula and how that processing is altered by experience. Findings at each level will be compared with the findings in mammalian systems that are obtained in other laboratories to see where the mormyrid studies can provide useful perspectives or insights into mammalian cerebellar function.