Cerebellar Circuitry and Plasticity in an Electric Fish

电鱼的小脑回路和可塑性

• 批准号: 6558466
• 负责人: VICTOR Z. HAN
• 金额: $ 20.15万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6558466
• 关键词: Osteichthyes; action potentials; biocytin; calcium ion; cerebellar Purkinje cell; cerebellum; efferent nerve; electrodes; electrophysiology; neural plasticity; neuroanatomy; neurophysiology; synapses; voltage /patch clamp

DESCRIPTION (provided by applicant): The neuronal circuitry of the cerebellum appears to be relatively simple and well defined, but the computation performed by this circuitry is not understood. In addition, forms of synaptic plasticity have been defined in the cerebellum that may be responsible for motor learning, but critical questions regarding this plasticity are still unanswered. The objective of the proposed research is to address fundamental issues cerebella circuitry and plasticity through a study of the unique cerebellum of mormyrid electric fish. Two features of the mormyrid cerebellum make it well suited to address these issues: 1) Purkinje cells and cerebellar output cells (cells of the cerebellar nuclei in mammals) are adjacent to each other in mormyrid fish, making possible experiments on integration and plasticity at the important synapse between these two cell types that can not be done in mammals. 2) The regions of termination for climbing fiber and parallel fiber inputs on Purkinje cells are distinct and well separated in the mormyrid but not in the mammal. This separation facilitates the determination of intrinsic differences between the two dendritic regions and the determination of how the two inputs interact to generate synaptic plasticity. The project has two Specific Aims: 1) to determine the basic synaptic and intrinsic physiology of Purkinje and cerebellar output cells in mormyrid electric fish; and 2) to determine the types of plasticity at parallel fiber and Purkinje cell synapses in mormyrid electric fish. The primary method will be whole-cell patch recording in vitro slices, combined with labeling of recorded elements for morphological identification.

描述（由申请人提供）：小脑的神经元电路似乎相对简单且定义明确，但该电路执行的计算尚不清楚。此外，小脑中突触可塑性的形式已被定义，可能负责运动学习，但有关这种可塑性的关键问题仍未得到解答。拟议研究的目的是通过研究斑尾电鱼独特的小脑来解决小脑电路和可塑性的基本问题。斑尾鱼小脑的两个特征使其非常适合解决这些问题：1）斑尾鱼中的浦肯野细胞和小脑输出细胞（哺乳动物小脑核细胞）彼此相邻，使得在这两种细胞类型之间的重要突触处进行整合和可塑性实验成为可能，而这在哺乳动物中是无法完成的。 2）浦肯野细胞上的攀爬纤维和平行纤维输入的终止区域在斑鸠中是不同的并且分离良好，但在哺乳动物中则不然。这种分离有助于确定两个树突区域之间的内在差异，以及确定两个输入如何相互作用以产生突触可塑性。该项目有两个具体目标：1）确定斑尾电鱼浦肯野和小脑输出细胞的基本突触和内在生理学； 2) 确定斑尾电鱼平行纤维和浦肯野细胞突触的可塑性类型。主要方法是体外切片全细胞贴片记录，结合记录元素的标记进行形态学识别。