Core Grant for Vision Research

视觉研究核心资助

• 批准号: 6945497
• 负责人: Curtis R Brandt
• 金额: $ 54.81万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6945497
• 关键词: biomedical facility; vision

DESCRIPTION (provided by applicant): This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules. These are Gene Delivery/Quantitative Molecular Biology,Pathology and Imaging, and Animal Models/ Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease. In addition, gene delivery to cells in culture is an essential research tool. The Gene Delivery Module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas will enhance the abilities of those researchers currently using these methods but more importantly, the core will make it easier for researchers currently not using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. These methodologies are also critical for studies using animal models. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assessing the effects of their agents, manipulations, and vectors/constructs on ocular function in rodents and primates. The services provided by these modules have been functioning for some time, but their ability to assist researchers has been limited by a lack of consistent support, and particularly by a lack of personnel. Funding of this proposal will enable us to improve our productivity and conduct work in a more timely manner, speeding the pace of progress in vision research on campus. It will allow us to expand the assistance available to eye researchers on campus and perhaps attract other investigators to eye research.

描述（由申请人提供）：这个P30核心补助金的视觉研究申请从威斯康星州视觉研究人员的大学提出了三个模块。这些是基因递送/定量分子生物学，病理学和成像，动物模型/眼器官培养。有效地将基因递送到细胞的能力是治疗眼部疾病的强大的新治疗方法。此外，向培养中的细胞递送基因是一种必不可少的研究工具。基因传递模块将构建载体，制备这些材料的高滴度储备供研究人员使用，并协助研究人员使用基因传递方法。定量方法在分子生物学中的应用越来越广泛。特别是通过RT-PCR定量基因表达和使用阵列技术分析基因表达变化。定量分子生物学模块将为这些领域提供专门的技术专长，将提高目前使用这些方法的研究人员的能力，但更重要的是，核心将使目前不使用这些技术的研究人员更容易将其添加到他们的研究计划中。随着对转化研究的重视，病理学分析和先进的成像方法变得越来越重要。这些方法对于使用动物模型的研究也至关重要。病理学和成像模块将提供先进的组织学、显微镜和图像分析，以及组织切片和培养细胞的形态测定。新疗法的开发和提高我们对视觉系统功能的理解依赖于将体外工作转移到动物模型上。动物模型和眼器官培养模块将帮助研究人员使用动物模型（灵长类动物和啮齿动物），实验性青光眼诱导，成像和眼生理学。这些专业知识将有助于核心用户评估其药剂、操作和载体/结构对啮齿动物和灵长类动物眼功能的影响。这些单元提供的服务已经运作了一段时间，但由于缺乏持续的支持，特别是缺乏人员，它们协助研究人员的能力受到限制。这项建议的拨款将使我们能够提高我们的生产力和更及时地开展工作，加快校园视觉研究的进展步伐。它将使我们能够扩大对校园眼科研究人员的援助，并可能吸引其他研究人员进行眼科研究。