TRANSGENIC & TARGETED MUTANT MICE PRESERVATION: BREEDING COLONIES: CRYOPRESERVE

转基因

• 批准号: 7153931
• 负责人: MURIEL T DAVISSON
• 金额: $ 32.89万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7153931
• 关键词: animal breeding; animal colony; cryopreservation; embryo /fetus preservation; flow cytometry; gene targeting; genetic registry /resource /referral center; genetic strain; genetically modified animals; genotype; germ free condition; inbreeding; information dissemination; laboratory mouse; molecular biology information system; mutant; phenotype; polymerase chain reaction; sperm; technology /technique development; tissue /organ preservation

DESCRIPTION (provided by applicant): This competing renewal seeks continued funding for The Jackson Laboratory's (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of genetically engineered mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific gene mutation (gene targeting), and chemical mutagenesis is providing new and powerful tools for biomedical research. The production and use of such mice has rapidly expanded, increasing the need for resource centers to maintain, preserve, and distribute them at a high health status and as free from legal restrictions as possible. Researchers frequently receive so many requests for their mice that they must be relieved of the burden that distributing mice places on their research programs and grants. TJL has maintained and distributed mutant strains of mice for over 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is evidenced by its rapid growth. Since it began, the IMR has accepted >950 mutant stocks, of which >300 are still being distributed from the breeding colony. The remaining strains have been archived as frozen embryos or sperm. Another 120 are currently in the importation process. In the first 4.5 years of the current grant period, the IMR distributed >450,000 mice; in 2002 mice were distributed to 750 investigators and supported research funded by nearly every NIH categorical institute. We request support for rederivation, cryopreservation, and strain development to improve the scientific value of IMR strains; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to (1) develop more reliable methods of sperm cryopreservation and recovery, (2) characterize the tissue- and organ-specific expression of Cre-expressing strains by using appropriate reporter strains already present in the IMR, and (3) carry out further phenotype characterization of selected mutants to enhance their value. IMR acceptance of strains is coordinated with the NCRR-funded Mutant Mouse Regional Resource Centers (MMRRC), the National Cancer Institute Mouse Models of Human Cancer Consortium (MMHCC), and the European Mutant Mouse Archive (EMMA) to avoid duplication of resources.

描述（由申请人提供）： 这项竞争性更新寻求继续为杰克逊实验室 (TJL) 的诱导突变资源 (IMR) 提供资金，以继续向科学界提供这一重要的基因工程小鼠资源。通过基因转移（转基因小鼠）、同源重组和时间和组织特异性基因突变（基因靶向）以及化学诱变选择性地改变小鼠基因组，为生物医学研究提供了新的强大工具。此类小鼠的生产和使用迅速扩大，增加了对资源中心维持、保存和分发它们的高度需求，使其保持良好的健康状态并尽可能不受法律限制。研究人员经常收到如此多的老鼠请求，因此他们必须摆脱分配老鼠给他们的研究项目和拨款带来的负担。 TJL 维护和分销小鼠突变品系已有 50 多年的历史。 1992年，TJL建立了IMR，其目的是进口、冷冻保存和分发具有生物医学重要性的诱导突变小鼠。 IMR 的快速增长证明了其需求。自成立以来，IMR 已接受超过 950 个突变体种群，其中超过 300 个仍在从繁殖群体中分发。其余菌株已作为冷冻胚胎或精子存档。另外 120 个目前正在进口过程中。在当前资助期的前 4.5 年中，IMR 分发了超过 450,000 只小鼠； 2002 年，小鼠被分发给 750 名研究人员，并支持几乎所有 NIH 分类研究所资助的研究。我们请求支持再衍生、冷冻保存和菌株开发，以提高IMR菌株的科学价值；小鼠的饲养是为了在尽可能收回成本的基础上分发给其他科学家。为了改进 IMR 资源计划，我们建议进行研究 (1) 开发更可靠的精子冷冻保存和恢复方法，(2) 通过使用 IMR 中已有的适当报告菌株来表征 Cre 表达菌株的组织和器官特异性表达，以及 (3) 对所选突变体进行进一步的表型表征，以提高其价值。 IMR 对品系的接受是与 NCRR 资助的突变小鼠区域资源中心 (MMRRC)、国家癌症研究所人类癌症模型小鼠模型联盟 (MMHCC) 和欧洲突变小鼠档案库 (EMMA) 协调进行的，以避免资源重复。