ASI-Spectral Karyotyping (SKY) System

ASI-光谱核型分析 (SKY) 系统

• 批准号: 6877535
• 负责人: MURIEL T DAVISSON
• 金额: $ 18.5万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877535
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; colorimetry; cytogenetics; karyotype; spectrometry

DESCRIPTION (provided by applicant): New genetic technologies combined with a well-characterized genome make the mouse the preferred mammalian model for understanding human diseases and identifying disease genes. Investigators must be able to characterize these models both physiologically and genetically to exploit their full biomedical potential. To realize this potential investigators must make the same basic clinical assessments used in human diagnostic medicine, often including chromosomal and histological analyses. The staff of The Jackson Laboratory (TJL) is at the forefront both in defining the normative biology of standard inbred mice and in characterizing hundreds of new spontaneous and induced mutations of biomedical significance; karyotypic analysis is an essential aspect of this characterization. TJL investigators currently rely on limited part-time cytogenetic services provided by Dr. Davisson's research personnel through her cytogenetic research program. This compromises both the productivity of her program and the research progress in programs requiring karyotypic analysis. Funding is requested for the purchase of a semi-automated spectral karyotyping system, the ASI-SKY System, Applied Spectral Imaging, Inc. Vista CA, that has proven its reliability in human diagnostic medicine and in basic research settings. This system, which also delivers quantitative spectral tissue analysis, represents a cost-efficient mechanism for cytogenetic assessment without requiring cytogenetic expertise, thus extending such analytical capabilities to all interested investigators. The ASI-SKY System will directly enhance the work of 17 TJL investigators with 34 NIH-funded grants who currently lack access to semi-automated, quantitative, digital spectral imaging instrumentation. The ASI-SKY system offers 4 capabilities unavailable at TJL: 1) semi-automated mouse chromosome karyotyping, 2) quantitative measurement of tissue-specific differences in mutant and control mice, 3) simultaneous spectroscopic assessment of multiple distinct proteins in the same tissue sample and 4) capture, analysis and simple colorimetric presentation of emission spectra from every pixel in an image. Delivery of technician-assisted, fee-for-service access to this state-of-the-art instrumentation by the Biological Imaging Service under the guidance of Dr. Davisson, an established cytogeneticist, will greatly enhance ongoing research efforts and assure maximum use of such instrumentation, thus providing a cost-effective way to fill an urgent, and currently unmet need of TJL Research Staff.

描述（由申请人提供）：新的基因技术与良好特征的基因组相结合，使小鼠成为了解人类疾病和识别疾病基因的首选哺乳动物模型。研究人员必须能够从生理学和遗传学上描述这些模型，以充分利用其生物医学潜力。为了实现这一潜力，研究人员必须进行与人类诊断医学相同的基本临床评估，通常包括染色体和组织学分析。杰克逊实验室（TJL）的工作人员在定义标准近亲繁殖小鼠的规范生物学和描述数百种具有生物医学意义的新自发和诱导突变方面处于最前沿；核型分析是这种特征的一个重要方面。TJL的研究人员目前依靠有限的兼职细胞遗传学服务，由Davisson博士的研究人员通过她的细胞遗传学研究计划提供。这既损害了她的程序的生产力，也损害了需要核型分析的程序的研究进展。申请资金用于购买半自动光谱核型系统ASI-SKY系统，应用光谱成像公司。Vista CA已经证明了它在人类诊断医学和基础研究环境中的可靠性。该系统还提供定量光谱组织分析，代表了一种成本效益高的细胞遗传学评估机制，无需细胞遗传学专业知识，从而将这种分析能力扩展到所有感兴趣的研究人员。ASI-SKY系统将直接加强17名TJL研究人员的工作，这些研究人员目前缺乏半自动、定量、数字光谱成像仪器。ASI-SKY系统提供了TJL无法提供的4项功能：1)半自动小鼠染色体核型；2)突变小鼠和对照小鼠组织特异性差异的定量测量；3)同一组织样品中多种不同蛋白质的同时光谱评估；4)图像中每个像素的发射光谱的捕获、分析和简单比色表示。在细胞遗传学家Davisson博士的指导下，由生物成像服务中心提供技术人员辅助的、按服务收费的最先进的仪器，将极大地加强正在进行的研究工作，并确保最大限度地利用这些仪器，从而提供一种具有成本效益的方式来填补TJL研究人员的紧急需求，目前尚未得到满足。