TRANSGENIC & TARGETED MUTANT MICE PRESERVATION: BREEDING COLONIES: CRYOPRESERVE

转基因

• 批准号: 7391976
• 负责人: MURIEL T DAVISSON
• 金额: $ 27.08万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391976
• 关键词: TRANSGENIC; TARGETED; MUTANT; MICE; PRESERVATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS This competing renewal seeks continued funding for The Jackson Laboratory''''''''s (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of transgenic and targeted mutation mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific production and use of induced mutant mice generated by these means has rapidly expanded, increasing the need for a resource to maintain, preserve, and distribute these mutant mice at a high health status and with minimal restrictions. Researchers frequently receive requests for mice they have created and want to believed of the burden that distributing mice to other scientists places on their research programs and grants. TJL, founded in 1929 as a non-profit research institution, has maintained and distributed mutant strains of mice for nearly 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is compelling and evidence by its rapid growth. Since it began, the IMR has accepted 495 mutant stocks, is distributing 299 of these, with another 58 currently in the importation process. In 1997, nearly 60,000 mice were distributed. We request support for rederivation, cryopreservation, and strain development, and strain development to improve the scientific value of strains in the IMR; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to: (1) develop methods to assure pathogen-free cyropreservation of mouse sperm, (2) phenotypically characterize new induced mutations and mutations transferred to new congenic genetic backgrounds, and (3) develop more cost-effective genotyping methods. Demand for IMR mice continues to increase, coincident with a decline in funding as several non-renewable start-up grants terminate. The ratio of rejected to accepted strains is increasing (see Introduction). The size of the need is beyond the scope of the NCRR alone to fund. NIAID and NIAMS already contribute to this effort by Intra-Agency Agreements. During the first 4 years of the current grant, over 70% of the mice supplied by the IMR were received by NIH intramural and extramural grantees of 16 NIH Institutes. To address the growing need we request approval of an expansion of this national resource for transgenic and targeted mice to authorize the NCRR to seek additional funding from NIH institutes. To aid in review we have subdivided the overall budget components. The Introduction explains the current funding mechanisms and our proposal to expand the IMR without an excessive burden to the NCRR.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。关于艾滋病，这项竞争性的更新为杰克逊实验室‘’‘’‘’‘’ （TJL）诱导突变资源（IMR）寻求持续的资金，以继续为科学界提供转基因和靶向突变小鼠的关键资源。通过基因转移（转基因小鼠）、同源重组以及通过这些方法产生的诱导突变小鼠的时间和组织特异性生产和使用来选择性改变小鼠基因组的研究迅速扩大，增加了对资源的需求，以维持、保存和分配这些突变小鼠，使其处于高度健康状态，并且限制最少。研究人员经常收到使用他们创造的老鼠的请求，并希望相信将老鼠分发给其他科学家会给他们的研究项目和资助带来负担。TJL成立于1929年，是一家非营利性研究机构，近50年来一直维持和分发小鼠突变株。1992年，TJL建立了IMR，其目的是导入、冷冻保存和分发具有重要生物医学意义的诱导突变小鼠。对国际货币基金组织的需求是令人信服的，其快速增长证明了这一点。自启动以来，国际遗传资源研究所已接收了495种突变品种，其中299种正在分发，另有58种目前正在进口过程中。一九九七年，共派发了近六万只老鼠。我们要求支持再衍生、冷冻保存和菌株开发，以及菌株开发以提高菌株在IMR中的科学价值；在尽可能多地回收成本的基础上，饲养老鼠分发给其他科学家。为了改善IMR资源计划，我们建议开展以下研究：(1)开发确保小鼠精子无病原体保存的方法；(2)对新的诱导突变和转移到新的先天性遗传背景的突变进行表型表征；(3)开发更具成本效益的基因分型方法。对IMR mice的需求继续增加，与此同时，由于一些不可续期的启动资助终止，资金减少。拒收菌株与接受菌株的比例正在增加（见引言）。需求的规模超出了NCRR单独提供资金的范围。NIAID和NIAMS已经通过机构内协议为这一努力作出了贡献。在目前拨款的前4年里，超过70%的由IMR提供的小鼠被16个NIH研究所的NIH校内和校外受助人接收。为了解决日益增长的需求，我们请求批准扩大转基因和靶向小鼠的国家资源，授权NCRR向NIH机构寻求额外的资金。为了便于审查，我们将总预算的组成部分进行了细分。导言部分解释了目前的筹资机制，以及我们在不给国家风险评估带来过度负担的情况下扩大国际风险评估的建议。