TRANSGENIC & TARGETED MUTANT MICE PRESERVATION: BREEDING COLONIES: CRYOPRESERVE

转基因

• 批准号: 7391976
• 负责人: MURIEL T DAVISSON
• 金额: $ 27.08万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391976
• 关键词: TRANSGENIC; TARGETED; MUTANT; MICE; PRESERVATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS This competing renewal seeks continued funding for The Jackson Laboratory''''''''s (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of transgenic and targeted mutation mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific production and use of induced mutant mice generated by these means has rapidly expanded, increasing the need for a resource to maintain, preserve, and distribute these mutant mice at a high health status and with minimal restrictions. Researchers frequently receive requests for mice they have created and want to believed of the burden that distributing mice to other scientists places on their research programs and grants. TJL, founded in 1929 as a non-profit research institution, has maintained and distributed mutant strains of mice for nearly 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is compelling and evidence by its rapid growth. Since it began, the IMR has accepted 495 mutant stocks, is distributing 299 of these, with another 58 currently in the importation process. In 1997, nearly 60,000 mice were distributed. We request support for rederivation, cryopreservation, and strain development, and strain development to improve the scientific value of strains in the IMR; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to: (1) develop methods to assure pathogen-free cyropreservation of mouse sperm, (2) phenotypically characterize new induced mutations and mutations transferred to new congenic genetic backgrounds, and (3) develop more cost-effective genotyping methods. Demand for IMR mice continues to increase, coincident with a decline in funding as several non-renewable start-up grants terminate. The ratio of rejected to accepted strains is increasing (see Introduction). The size of the need is beyond the scope of the NCRR alone to fund. NIAID and NIAMS already contribute to this effort by Intra-Agency Agreements. During the first 4 years of the current grant, over 70% of the mice supplied by the IMR were received by NIH intramural and extramural grantees of 16 NIH Institutes. To address the growing need we request approval of an expansion of this national resource for transgenic and targeted mice to authorize the NCRR to seek additional funding from NIH institutes. To aid in review we have subdivided the overall budget components. The Introduction explains the current funding mechanisms and our proposal to expand the IMR without an excessive burden to the NCRR.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。关于艾滋病，这一竞争更新寻求继续为杰克逊实验室S(TJL)诱导突变资源(IMR)继续提供资金，以继续向科学界提供这一关键的转基因和定向突变小鼠资源。通过基因转移(转基因小鼠)、同源重组以及通过这些方法产生的诱导突变小鼠的时间和组织特异性生产和使用来选择性地改变小鼠基因组的方法迅速扩大，增加了对资源的需求，以维持、保存和分发这些处于高健康状态和最小限制的突变小鼠。研究人员经常收到关于他们创造的小鼠的请求，并希望相信将小鼠分发给其他科学家会给他们的研究计划和拨款带来负担。TJL成立于1929年，是一家非营利性研究机构，近50年来一直在维护和分发突变小鼠品系。1992年，TJL建立了IMR，其目的是进口、冷冻保存和分发具有生物医学意义的诱导突变小鼠。IMR的快速增长证明了对IMR的需求是令人信服的。自开始以来，IMR已经接受了495个突变种群，正在分发其中的299个，另有58个目前正在进口过程中。1997年，分发了近6万只老鼠。我们请求支持重新衍生、低温保存、菌株开发和菌株开发，以提高IMR中菌株的科学价值；在尽可能多的成本回收的基础上，维持小鼠以分发给其他科学家。为了改进IMR资源计划，我们建议进行以下研究：(1)开发确保小鼠精子无病原体冷冻保存的方法；(2)对新的诱导突变和转移到新的同源遗传背景的突变进行表型表征；(3)开发更具成本效益的基因分型方法。对IMR MICE的需求继续增加，与此同时，随着几笔不可续期的启动赠款终止，资金减少。被拒绝的菌株与被接受的菌株的比例正在增加(见导言)。这一需求的规模超出了NCRR单独提供资金的范围。NIAID和NIAMS已经通过机构内协议为这一努力做出了贡献。在当前赠款的前4年，IMR提供的小鼠中有70%以上是由16个NIH研究所的NIH内外受赠者接收的。为了满足日益增长的需求，我们请求批准扩大这一转基因和靶向小鼠的国家资源，以授权NCRR从NIH研究所寻求额外的资金。为协助审查，我们对总体预算部分进行了细分。引言解释了目前的筹资机制和我们提出的扩大IMR而不给NCRR带来过多负担的建议。