CORE--ANALYTICAL

这项服务核心的总体目标是向所有其他项目提供准确和及时的数据。不需要开发新的方法来完成这项任务。该服务核心将负责所有项目的一套通用衡量标准。将完成对项目1和2中的铁、TIBC、TSAT和铁蛋白的血清分析。尿样将使用标准方法用原子吸收光谱仪进行铁分析。尿液和血浆中的异前列腺素将作为氧化应激的标志物进行监测。在动物中，将评估项目3、4和核心C中的血红蛋白、红细胞压积、血浆铁、TIBC、TSAT和肝脏非血红素铁含量。目的1为测定血浆和尿液中的铁和铁相关蛋白以及从受试者的尿样和血浆中测定氧化应激标志物异前列腺素提供实验室支持。 目的：测定RI品系小鼠脑组织和脑组织铁含量。

The overall goal of this service Core is to provide accurate and timely data to all other Projects. There are no new methodologies that need to be developed to accomplish this task. This service Core will be responsible for a set of common measurement for all Projects. Analysis of sera from Projects 1 & 2 for iron, TIBC, TSAT, and ferritin will be completed. Urine samples will be analysed for iron by atomic absorption spectrophometry using standard methods. Urine and plasma isoprostanes will be monitored as markers of oxidative stress. In the animals, hemoglobin, hematocrit, plasma iron, TIBC, TSAT, and liver non-heme iron content will be assessed for Projects 3, 4 and Core C. Aim 1 To provide laboratory support for the determination of iron and iron-related proteins in plasma and urine and for the determination of isoprostanes, which are markers of oxidative stress, in urine and plasma from sample from human subjects. Aim 2: To determine the levels of tissue and brain iron in RI strains of mice.