CORE--ANALYTICAL

该服务核心的总体目标是为所有其他项目提供准确和及时的数据。不需要开发新的方法来完成这项任务。该服务核心将负责所有项目的一套通用测量。将完成项目1和2的血清铁、TIBC、TSAT和铁蛋白分析。将使用标准方法通过原子吸收分光光度法分析尿样中的铁。将监测尿液和血浆异前列腺素作为氧化应激的标志物。在动物中，将评估项目3、4和核心C的血红蛋白、红细胞压积、血浆铁、TIBC、TSAT和肝脏非血红素铁含量。目的1为血浆和尿液中铁及铁相关蛋白的测定以及尿液和血浆中氧化应激标志物异前列腺素的测定提供实验室支持。 目的2：测定RI小鼠组织和脑铁含量。

The overall goal of this service Core is to provide accurate and timely data to all other Projects. There are no new methodologies that need to be developed to accomplish this task. This service Core will be responsible for a set of common measurement for all Projects. Analysis of sera from Projects 1 & 2 for iron, TIBC, TSAT, and ferritin will be completed. Urine samples will be analysed for iron by atomic absorption spectrophometry using standard methods. Urine and plasma isoprostanes will be monitored as markers of oxidative stress. In the animals, hemoglobin, hematocrit, plasma iron, TIBC, TSAT, and liver non-heme iron content will be assessed for Projects 3, 4 and Core C. Aim 1 To provide laboratory support for the determination of iron and iron-related proteins in plasma and urine and for the determination of isoprostanes, which are markers of oxidative stress, in urine and plasma from sample from human subjects. Aim 2: To determine the levels of tissue and brain iron in RI strains of mice.