Poliovirus Receptor and Poliovirus Pathogenesis

脊髓灰质炎病毒受体和脊髓灰质炎病毒发病机制

• 批准号: 6920201
• 负责人: Eckard Wimmer
• 金额: $ 13.43万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6920201
• 关键词: dynein ATPase; gastrointestinal infection; genetically modified animals; gut associated lymphoid tissue; laboratory mouse; microtubules; neurotropic virus; pathologic process; poliovirus; tissue /cell culture; virus infection mechanism; virus receptors

DESCRIPTION (provided by applicant): Following ingestion and entry into the gastrointestinal (Gl) tract, poliovirus, a human neurotropic enterovirus of Picornaviridae, may replicate in gastrointestinal-associated lymphatic tissues (GALT) and, subsequently, spread to the systemic circulation and to the CNS. Numerous attempts to study oral poliovirus infection in transgenic mice have so far failed. A focus of this application is to construct a novel transgenic mouse model for oral poliovirus infection that can be used to determine the site(s) of poliovirus proliferation in the Gl tract. A second focus of this application is to study mechanism(s) by which poliovirus is migrating in polarized tissue culture cells, in cells of neuronal origin, and in axons. Experiments have been developed based on our hypothesis that an interaction between the poliovirus receptor CD155 and Tctex-1, a cargo binding polypeptide of the dynein motor complex, is responsible for movement of poliovirus along microtubules.

性状（由申请方提供）：脊髓灰质炎病毒（一种小核糖核酸病毒科的人类嗜神经性肠道病毒）摄入并进入胃肠道（GI）后，可能在胃肠相关淋巴组织（GALT）中复制，随后扩散至体循环和CNS。 迄今为止，许多研究转基因小鼠口服脊髓灰质炎病毒感染的尝试都失败了。 本申请的焦点是构建用于口服脊髓灰质炎病毒感染的新型转基因小鼠模型，其可用于确定胃肠道中脊髓灰质炎病毒增殖的位点。 本申请的第二个重点是研究脊髓灰质炎病毒在极化的组织培养细胞、神经元来源的细胞和轴突中迁移的机制。 我们的假设是脊髓灰质炎病毒受体CD 155和Tctex-1（动力蛋白运动复合物的货物结合多肽）之间的相互作用负责脊髓灰质炎病毒沿着微管的运动，基于这一假设已经开展了实验。