Percutaneous Devices Permitting Skin Cell Attachment

描述（由申请人提供）：经皮器械在患者护理中发挥重要作用。然而，与穿透皮肤相关的感染风险并非无关紧要。经皮装置用于多种功能，如透析、化疗、假肢附件和经皮传感器（葡萄糖）。仅中心静脉导管每年就与80,000至250,000例血流感染有关，每次感染的相关死亡率为12%-25%，每次感染的费用为28,000美元。本研究的目的是创造一个界面，促进表皮和真皮细胞与经皮医疗器械表面的整合。我们相信，通过细胞整合到经皮医疗器械中来愈合皮肤伤口，将提供一个密封，防止细菌附着和随后的生物膜形成。这一创新可能会防止与这些设备相关的严重并发症的最常见原因。与NIH路线图一致，拟议的研究将由一个跨学科研究团队进行，包括皮肤生物学家，来自两个NSF工程研究中心的研究人员，一个具有先进生物材料的专业知识，另一个具有生物膜研究的专业知识，以及开发和销售用于血管内通路的经皮设备的工业合作伙伴。具体目标如下：1.加强工作。开发和表征允许在经皮装置穿透皮肤的地方附着皮肤细胞的生物材料。2. 研究体外和体内皮肤对优化的生物材料的反应（生物整合）的细胞和分子事件。3. 评估细菌附着和生物膜形成作为皮肤/生物材料整合的功能。

DESCRIPTION (provided by applicant): Percutaneous devices play an essential role in patient care. However, the risks of infections associated with penetrating the skin are not inconsequential. Percutaneous devices are used for such diverse functions as dialysis, chemotherapy, attachments for prosthetic limbs and percutaneous sensors (glucose). Central venous catheters alone are associated with an estimated 80,000 to 250,000 blood stream infections annually, with an associated mortality of 12%-25% for each infection and a cost of >$28,000 per episode. The goal of this research is to create an interface that promotes epidermal and dermal cell integration with the surface of percutaneous medical devices. We believe that healing the cutaneous wound with cellular integration into percutaneous medical devices will provide a seal preventing bacterial attachment and subsequent biofilm formation. This innovation may prevent the most common cause of serious complications associated with these devices. Consistent with The NIH Roadmap, the proposed research will be conducted by an interdisciplinary research team including, skin biologists, researchers from two NSF Engineering Research Centers, one with expertise in advanced biomaterials, the other with expertise in the study of biofilm, as well as an industrial partner that develops and markets percutaneous devices for intravascular access. We will carry out the following Specific Aims: 1. Develop and characterize biomaterials that permit attachment of skin cells where percutaneous devices penetrate the skin. 2. Study the cellular and molecular events of the in vitro and in vivo skin responses (biointegration) to optimized biomaterials. 3. Evaluate bacterial attachment and biofilm formation as a function of skin/biomaterial integration.