Percutaneous Devices Permitting Skin Cell Attachment-Inhibiting Biofilm Formation

描述(由申请人提供)： 这项研究的目标是提高皮肤科培训中对研究事业感兴趣的优秀内科医生的基础知识和研究技能。该项目利用学员以前的教育经验，并将其纳入一个参与NIBIB资助的R01的跨学科研究团队，其中包括皮肤生物学家、来自两个NSF工程研究中心的研究人员，一个拥有先进生物材料的专业知识，另一个拥有生物膜研究的专业知识，以及开发和营销用于血管内进入的经皮设备的工业合作伙伴。经皮穿刺器在患者护理中起着至关重要的作用；它们用于各种功能，如透析、化疗、假肢附着物和经皮传感器(葡萄糖)。然而，与穿透皮肤相关的感染风险并不是微不足道的。据估计，仅中心静脉导管每年就与8万至25万例血流感染有关，每次感染的相关死亡率为12%-25%，每一次感染的成本为28,000美元。赠款的目标是创造一种界面，促进表皮和真皮细胞与经皮医疗设备表面的整合，同时最大限度地减少生物膜的形成。我们相信，通过将细胞整合到经皮医疗设备中来愈合皮肤伤口将提供一种密封，防止细菌附着和随后的生物膜形成。受训者将执行评估细菌附着和生物膜形成作为皮肤/生物材料集成功能的具体目标。培训将对他的研究事业发展做出重大贡献，从这项工作中获得的信息将有助于防止与这些设备相关的严重并发症的最常见原因。

DESCRIPTION (provided by applicant): The goal of this research is to enhance the fundamental knowledge and research skills of an excellent physician in dermatology training who is interested in a research career. This project takes advantage of the trainee's previous educational experience and incorporates him into an interdisciplinary research team participating in a NIBIB-funded R01, including skin biologists, researchers from two NSF Engineering Research Centers, one with expertise in advanced biomaterials, the other with expertise in the study of biofilm, as well as an industrial partner that develops and markets percutaneous devices for intravascular access. Percutaneous devices play an essential role in patient care; they are used for such diverse functions as dialysis, chemotherapy, attachments for prosthetic limbs and percutaneous sensors (glucose). However, the risks of infections associated with penetrating the skin are not inconsequential. Central venous catheters alone are associated with an estimated 80,000 to 250,000 blood stream infections annually, with an associated mortality of 12%-25% for each infection and a cost of >$28,000 per episode. The goal of the grant is to create an interface that promotes epidermal and dermal cell integration with the surface of percutaneous medical devices while minimizing biofilm formation. We believe that healing the cutaneous wound with cellular integration into percutaneous medical devices will provide a seal preventing bacterial attachment and subsequent biofilm formation. The trainee will carry out the Specific Aim to evaluate bacterial attachment and biofilm formation as a function of skin/biomaterial integration. The training will contribute significantly to his research career development, and information gained from this work will help prevent the most common cause of serious complications associated with these devices.