Fiber Optic Surface Plasmon Resonance Sensor

描述（由申请人提供）： 该项目的目标是开发一种通用的模块化光纤传感器系统，能够在体外量化特定蛋白质的生物学相关水平。该传感器系统专为快速床边分析、农村或技术落后环境中的初步诊断以及现场急救人员的及时诊断而设计。该传感器系统将比现有的市售可现场传感器更快，同时保持目标蛋白常见实验室测试的灵敏度和选择性。通过在光纤上构建传感器，传感器系统可以采用廉价、可互换的传感元件进行特定测试，并通过简单的自动化程序来准备传感元件进行操作。 这些传感器利用光纤上的表面等离子共振 (SPR) 通过免疫测定来检测标记物，并封装在薄膜聚合物外壳中，以防止传感区域的细胞污染。噬菌体展示将用于收集改进的蛋白质特异性受体，其具有目标应用所需的结合敏感性。由于测定是在光纤表面进行的，因此探针可以用作体外浸入式探针。我们选择了四个目标示例应用作为开发和优化传感器系统的基础：用于检测和确定心肌梗塞严重程度的蛋白质、用于评估伤口愈合能力的蛋白质、与缺血性中风相关的蛋白质；用于检测脊髓运动萎缩的蛋白质。传感器优化将针对发现这些蛋白质的特定生物基质。选择这些示例应用程序是为了演示传感系统的灵活性和相关性，但不应被视为其使用的限制情况。事实上，基于抗体的免疫测定方法的应用远远超出了这里考虑的范围。

DESCRIPTION (provided by applicant): The goal of this project is to develop a general, modular fiber optic sensor system capable of quantifying biologically relevant levels of specific proteins in vitro. The sensor system is designed for rapid bedside analysis, for primary diagnoses in rural or technologically poor environments, and timely diagnoses by first responders in the field. The sensor system will be faster than existing commercially available fieldable sensors while maintaining the sensitivity and selectivity of common laboratory tests for the target proteins. By constructing the sensors on optical fibers, the sensor system can employ inexpensive, interchangeable sensing elements for specific tests with simple automated procedures for preparing the sensing elements for operation. The sensors use surface plasmon resonance (SPR) on optical fibers to detect the markers through immunoassays, and are encapsulated in a thin film polymer housing that prevents cellular fouling of the sensing region. Phage display will be employed to collect improved protein specific receptors with desired binding sensitivities for target applications. Because the assay is conducted on the surface of an optical fiber, the probes can be used as immersion probes in vitro. Four target example applications around which we will base development and optimization of the sensor system have been chosen: proteins for detecting and determining the severity of myocardial infarctions, proteins for assessing ability of wounds to heal, proteins associated with ischemic stroke; proteins for detecting spinal motor atrophy. Sensor optimization will address specific biological matrices where these proteins are found. These example applications are selected to demonstrate the flexibility and relevancy of the sensing system, but should not be construed as the limiting cases of its use. In fact, the antibody-based immunoassay approach has applications far beyond those considered here.