MODULAR SURFACE PLASMON RESONANCE BIOSENSOR FOR IN-VITRO DETERMINATION OF PROTEIN

用于蛋白质体外测定的模块化表面等离激元共振生物传感器

• 批准号: 7835812
• 负责人: Karl S. Booksh
• 金额: $ 37.04万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2011-10-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7835812
• 关键词: Accident and Emergency department; Alzheimer' s Disease; Antibodies; Area; Arizona; Biological Assay; Biological Markers; Biosensor; Burn injury; Caliber; Cardiology; Caring; Cells; Chemistry; Chronic; Clinic; Clinical; Clinical Research; Complex Mixtures; Delaware; Detection; Diabetic ulcer; Diagnosis; Economically Deprived Population; Elements; Environment; Gold; Head; Health Personnel; Hospitals; In Vitro; Injury; Label; Laboratories; Location; Medical; Methods; Microfluidics; Modeling; Monitor; Myocardial Infarction; Optics; Patients; Peptides; Performance; Physiological; Procedures; Proteins; Protocols documentation; Refractive Indices; Resolution; Rural; Sampling; Serum; Signal Transduction; Spectrum Analysis; Stroke; Surface Plasmon Resonance; System; Testing; Treatment Efficacy; Triage; Whole Blood; Work; Wound Healing; base; cost; design; diabetic; emergency service responder; experience; flexibility; knowledge base; meetings; micro-total analysis system; multidisciplinary; nanoparticle; operation; sensor; stability testing

This project combines electrokinetic (EK) methods with surface plasmon resonance (SPR) spectroscopy to construct a flexible, modular lab-on-a-chip platform for determination of target biomarkers. Small volume channels are etched into a dielectric substrate where EK methods for concentration and separation (i.e. dielectrophoresis and electrophoretic capture) of target analytes from the other compounds in the biofluids are employed to condition the sample for SPR detection. For example dielectrophoresis and electrophoretic capture will be employed to locally concentrate the target analytes in the microfluidic channel while minimizing the local concentration of interfering proteins. Gold sensing pads can be employed for direct label-free SPR analyses, coated with bioreceptors to increase sensitivity and selectivity, or coated with bioreceptors for the analysis followed by signal amplification with the another antibody to the target biomarker. Methods for signal enhancement such as antibody sandwich assays will be included with the lab-on-a-chip design to achieve a greater degree of sensitivity. This sensor will be applied to detection of biomarkers for myocardial infarction. The proposed platform offers the following attributes beneficial to any bioassay: • The modular, 'chip' based sensing platform can separate, concentrate, and quantify multiple protein and peptide biomarkers required for a panel assay. • Electrokinetic methods and SPR detection with sandwich assays are both appropriate for analyzing physiological levels of proteins and peptides. • The system is ideally suited for small volume analyses. EP and DEP work best in small channels approximately 10 to 100 microns in diameter. SPR is sensitive to refractive index changes within 200 nm of the gold sensing pads. • EK can rapidly separate and concentrate analytes from complex mixtures based on electrophoretic mobility. High resolution separations are possible and 1000-fold preconcentration can be achieved with dielectrophoresis or addressable electrophoretic capture.

该项目结合了电动力学（EK）方法和表面等离子体共振（SPR）光谱，构建了一个灵活的模块化芯片实验室平台，用于测定目标生物标志物。在介质衬底上蚀刻小体积通道，EK方法用于目标分析物的浓度和分离（即介电电泳和电泳捕获）

项目成果

Dielectrophoretic mobility determination in DC insulator-based dielectrophoresis.

基于直流绝缘体的介电体现型的介电性迁移率。

• DOI: 10.1002/elps.201100034
• 发表时间: 2011-09
• 期刊: ELECTROPHORESIS
• 影响因子: 2.9
• 作者: Weiss, Noah G.;Jones, Paul V.;Mahanti, Prasun;Chen, Kang P.;Taylor, Thomas J.;Hayes, Mark A.
• 通讯作者: Hayes, Mark A.

Isoelectric focusing in a drop.

等电聚焦在一滴中。

• DOI: 10.1021/la104085t
• 发表时间: 2011
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: Weiss,NoahG;Hayes,MarkA;Garcia,AntonioA;Ansari,RafatR
• 通讯作者: Ansari,RafatR

Examining serum amyloid P component microheterogeneity using capillary isoelectric focusing and MALDI-MS.

• DOI: 10.1002/pmic.201000310
• 发表时间: 2011-01
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Weiss, Noah G.;Jarvis, Jason W.;Nelson, Randall W.;Hayes, Mark A.
• 通讯作者: Hayes, Mark A.

Improved detectability and signal strength for rotating phase fluorescence immunoassays through image processing.

通过图像处理提高旋转相荧光免疫测定的可检测性和信号强度。

• DOI: 10.1039/c0an00549e
• 发表时间: 2011
• 期刊: The Analyst
• 作者: Mahanti,Prasun;Taylor,Thomas;Hayes,MarkA;Cochran,Douglas;Petkus,MatthewM
• 通讯作者: Petkus,MatthewM

Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice.

• DOI: 10.1002/elps.201100622
• 发表时间: 2012-04
• 期刊: ELECTROPHORESIS
• 影响因子: 2.9
• 作者: Kenyon, Stacy M.;Weiss, Noah G.;Hayes, Mark A.
• 通讯作者: Hayes, Mark A.