Fiber Optic Surface Plasmon Resonance Sensor

描述(由申请人提供)： 该项目的目标是开发一种通用的、模块化的光纤传感器系统，能够在体外定量特定蛋白质的生物相关水平。该传感器系统设计用于快速床边分析，在农村或技术落后的环境中进行初步诊断，并由现场急救人员及时诊断。该传感器系统将比现有的商用可现场传感器更快，同时保持对目标蛋白质的常见实验室测试的灵敏度和选择性。通过在光纤上构建传感器，传感器系统可以使用廉价的、可互换的传感元件进行特定测试，并具有简单的自动化程序来准备用于操作的传感元件。 传感器使用光纤上的表面等离子体共振(SPR)通过免疫分析来检测标记，并封装在薄膜聚合物外壳中，以防止传感区域的细胞污染。噬菌体展示将被用来收集具有所需结合敏感性的改进的蛋白质特异性受体，以用于靶向应用。由于检测是在光纤表面进行的，因此该探针可以在体外用作浸没式探针。我们选择了四个目标实例应用来作为传感器系统开发和优化的基础：用于检测和确定心肌梗死严重程度的蛋白质、用于评估伤口愈合能力的蛋白质、与缺血性中风相关的蛋白质、用于检测脊髓运动性萎缩的蛋白质。传感器优化将解决这些蛋白质所在的特定生物基质。选择这些示例应用程序是为了证明传感系统的灵活性和相关性，但不应将其解释为其使用的限制情况。事实上，基于抗体的免疫分析方法的应用远远超出了这里考虑的范围。

DESCRIPTION (provided by applicant): The goal of this project is to develop a general, modular fiber optic sensor system capable of quantifying biologically relevant levels of specific proteins in vitro. The sensor system is designed for rapid bedside analysis, for primary diagnoses in rural or technologically poor environments, and timely diagnoses by first responders in the field. The sensor system will be faster than existing commercially available fieldable sensors while maintaining the sensitivity and selectivity of common laboratory tests for the target proteins. By constructing the sensors on optical fibers, the sensor system can employ inexpensive, interchangeable sensing elements for specific tests with simple automated procedures for preparing the sensing elements for operation. The sensors use surface plasmon resonance (SPR) on optical fibers to detect the markers through immunoassays, and are encapsulated in a thin film polymer housing that prevents cellular fouling of the sensing region. Phage display will be employed to collect improved protein specific receptors with desired binding sensitivities for target applications. Because the assay is conducted on the surface of an optical fiber, the probes can be used as immersion probes in vitro. Four target example applications around which we will base development and optimization of the sensor system have been chosen: proteins for detecting and determining the severity of myocardial infarctions, proteins for assessing ability of wounds to heal, proteins associated with ischemic stroke; proteins for detecting spinal motor atrophy. Sensor optimization will address specific biological matrices where these proteins are found. These example applications are selected to demonstrate the flexibility and relevancy of the sensing system, but should not be construed as the limiting cases of its use. In fact, the antibody-based immunoassay approach has applications far beyond those considered here.