Core--Immune monitoring

免疫监测核心将由杜克人类疫苗研究所（HVI）内的两个组成实验室提供的技术和服务组成：流式细胞术设施和免疫重建设施。RadCCORE免疫监测核心（核心C）将定期为研究人员提供高质量，最先进的流式细胞术，蛋白质阵列和基因表达测定支持，以支持他们的基础和应用研究工作。除了提供这些服务，核心将有助于开发新的方法，并使其提供给RadCCORE调查人员。Core C的三个目标是：1）为杜克RadCCORE进行的基础研究或转化研究提供最先进的多色荧光激活细胞分选和分析支持 investigators.这包括在我们的8色BD FACSVantage SE、7色BD LSRII、4色FACSCalibur和13色BD FACSAria上对细胞亚群进行高速分选和多色分析。2)使用我们的Bioscience Luminex微珠阵列阅读器提供生物样品（例如细胞因子、趋化因子、激素、信号转导蛋白）的多重蛋白阵列分析。3)在我们的四色实时BioRad Optical I-Cycler上为研究者提供定量实时PCR多重基因表达分析。特异性测定包括T细胞受体切除环定量以监测胸腺生成（qPCR），以及在单个样品中同时定量多达四种靶mRNA种类（qRT-PCR）。这三个目标的关键是我们致力于不断开发新的检测方法，以满足RadCCORE的需求。 investigators.通过教育和与核心用户群合作，我们将开发和优化他们的特定检测，并更有效地利用核心设施提供的最先进的仪器。

The Immune Monitoring Core will be comprised of technologies and services offered by two component laboratories within the Duke Human Vaccine Institute (HVI): the Flow Cytometry Facility and the Immune Reconstitution Facility. The RadCCORE Immune Monitoring Core (Core C) will routinely provide investigators with high quality, state-of-the-art flow cytometry, protein array, and gene expression assay support for their basic and applied research efforts. In addition to providing these services, the Core will be instrumental in developing new methodologies and making them available to RadCCORE Investigators. The three goals of Core C are: 1) Provide state-of-the-art, multi-color, fluorescence activated cell sorting and analytical support for basic research or translational investigations conducted by Duke RadCCORE investigators. This includes high-speed sorting and multi-color analysis of cellular subsets on our 8-color BD FACSVantage SE, 7-color BD LSRII, 4-color FACSCalibur and 13-color BD FACSAria. 2) Provide multiplex protein array profiling of biological samples (e.g. cytokines, chemokines, hormones, signaling transduction proteins) using our BioPlex Luminex bead array reader. 3) Provide quantitative real-time-PCR multiplex gene expression analysis to investigators on our 4-color real-time BioRad Optical I-Cycler. Specific assays include T cell receptor excision circle quantitation to monitor thymopoiesis (qPCR), and simultaneous quantitation of up to four target mRNA species in a single sample (qRT-PCR). Critical to all three of these aims will be our commitment to ongoing development of new assays to meet the needs of the RadCCORE investigators. By educating and working in collaboration with the Core User base we will develop and optimize their specific assays and more effectively utilize the state-of-the-art instrumentation offered by the Core Facility.