Core 2: Biocontainment and Immune Monitoring Core

核心2：生物防护和免疫监测 摘要 该计划的总体目标是开发一种或多种疫苗， betaCoV爆发。为了支持这一总体目标，生物防护和免疫监测核心 都集中在杜克核心由通过杜克提供的技术和服务组成 区域生物污染实验室（杜克RBL）、杜克人类疫苗研究所（DHVI）流式细胞术 和细胞分选设施，以及Montefiori和Ferrari抗体表征实验室。杜克RBL 是NIH建造的12个RBL之一，并且是第一个于2007年开始运行的RBL。杜克RBL提供了一个 安全、可靠、合规的BSL 2 -3/ABSL 2 -3和选择代理研究活动的行政保护伞 在杜克大学。杜克RBL拥有最先进的BSL 3生物防护实验室模块， 容纳单元分选，服务单元的组合（i. e.微生物学、病毒学、免疫学和动物学 模型支持），一个集成的ABSL 3动物园，以支持小动物攻毒模型， 旨在支持开发新出现/再出现感染的诊断、治疗和疫苗 和生物防御核心将定期为项目调查人员提供高包容性细胞分选， 综合表型分析、多重生物标志物分析、病毒载量、中和和抗体依赖性 细胞毒性测定和小动物SARS-CoV-2攻击模型。对所有五个核心至关重要 目标/任务是对仪器和测定进行持续质量控制的承诺;以及对 预测并满足所有四个项目和NHP核心的需求。通过教育和工作， 与项目研究人员合作，我们将优化分析，以满足他们的需求， 利用最先进的仪器和生物防护设施， 免疫监测核心由一个团队进行全面和集中的免疫监测， 跟踪记录将为所有拟议的研究增加价值，并将使项目调查人员能够更好地 高效地设计和开发泛β CoV疫苗。

Core 2: Biocontainment and Immune Monitoring Abstract The overall goal of this program is to develop a vaccine or vaccines that will be available when the next betaCoV outbreak occurs. In support of this overall goal, a Biocontainment and Immune Monitoring Core has been centralized at Duke. The Core is comprised of technologies and services offered through the Duke Regional Biocontanment Laboratory (Duke RBL), the Duke Human Vaccine Institute (DHVI) Flow Cytometry and Cell Sorting facility, and the Montefiori and Ferrari antibody characterization laboratories. The Duke RBL is one of 12 NIH-constructed RBLs, and was the first to begin operation in 2007. The Duke RBL provides an administrative umbrella for safe, secure and compliant BSL2-3/ABSL2-3 and Select Agent research activities at Duke University. The Duke RBL has state-of-the-art BSL3 biocontainment laboratory modules, high- containment cell sorting, a portfolio of service units (i. e. Microbiology, Virology, Immunology and Animal Models Support), an integrated ABSL3 vivarium to support small animal challenge models, and was purpose built to support the development of diagnostics, therapeutics and vaccines for emerging/reemerging infections and biodefense. The Core will routinely provide program investigators with high-containment cell sorting and comprehensive phenotyping, multiplex biomarker profiling, viral load, neutralization and antibody-dependent cellular cytotoxicity assays, and small animal SARS-CoV-2 challenge models. Critical to all five Core Aims/Tasks is a commitment to ongoing quality control of instruments and assays; and a strong commitment to anticipate and meet the needs of all four projects and the NHP Core. By educating and working in collaboration with program investigators we will optimize assays to meet their needs and more effectively utilize the state-of-the-art instrumentation and biocontainment facilities available through the Biocontaniment and Immune Monitoring Core. Comprehensive and centralized immune monitoring by a team with a proven track-record will add value to all proposed studies, and will allow project investigators to better, and more efficiently, design and develop panbetaCoV vaccines.