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BSL3 Flow Biomarker and Imaging

BSL3 流式生物标志物和成像

基本信息

批准号：
8234182
负责人：
Gregory D Sempowski
金额：
$ 24.39万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-03-01 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8234182
关键词：
Animal Model Animals Applied Research Area Basic Science Biological Biological Assay Biological Markers Bioluminescence Blood Cell Separation Cells Collaborations Complete Blood Count Computer Workstations Containment Core Facility Development Diagnostic Emergency Situation Emerging Communicable Diseases Fee-for-Service Plans Flow Cytometry Fluorescence Funding Future Goals Hematology Host Defense Human Image Imaging Techniques Immune response Immunologic Monitoring Immunophenotyping Infection Institutes Investigation Laboratories Leadership Life Monitor National Institute of Allergy and Infectious Disease Natural Immunity Pathogenicity Preparation Procedures Protein Array Protein Array Analysis Qualifying Reader Research Activity Research Personnel Running Sampling Secure Services Specimen Technology Tissues Training Training Activity Universities Vaccines adaptive immunity base biodefense biothreat cost effective cost efficient drug development in vivo insight meetings member novel therapeutics operation pathogen research facility

项目摘要

The overall objective of this core proposal is to establish a safe, secure and comprehensive Biosafety Level - 3 (BSL3) host-pathogen interaction core to support and enhance the activities of the SERCEB/RCE investigators. The core will provide state-of-the-art flow cytometry and cell sorting, multi-modal live animal imaging, and multiplex biomarker analysis for SERCEB or other RCE investigators running studies in the Regional Biocontainment Laboratory at Duke or for member investigators that send specimens to the core for analysis. To accomplish this goal we have available four base technologies in BSL3 containment: a BDFACS Aria cell sorter, an IVIS-Xenogen Spectrum imager, a BibRad luminex bead array reader and an Abbott Cell Dyn 3700 veterinary hematology analyzer. In support of these base technologies we have offline-computer workstations outside containment, dedicated BSL2 and BSL3 laboratory space for sample preparation, highly trained technical staff in BSL3 and ABSL3 procedures and a PI and Co-investigator with an established track record in immune monitoring research and core facility management. These core technologies are contained at BSL3 in the NIAID-RBL at Duke and run as a core in collaboration with the proposed SERCEB Aerobiology/Animal Models core. This will allow comprehensive host-pathogen analysis at BSL3 under appropriate Standard Operating Procedures for SERCEB/RCE investigators. The use of flow cytometry and in vivo imaging techniques will allow us to monitor both host response and the course of infection, replication, persistence, and dissemination of priority pathogens. Blood and tissue biomarker analysis will provided further valuable insight into host-pathogen interactions which will only enhance the challenge and mechanistic studies proposed by projects in the SERCEB consortium. Areas of active biomedical investigation to be supported by this host-pathogen interation core include fundamental pathogenicity of Priority and Select Agent pathogens, innate and adaptive immunity and host'defense, and development of drugs, diagnostics and vaccines for identified bio-threat pathogens.

本核心提案的总体目标是建立一个安全、可靠和全面的生物安全水平