BSL3 Flow Biomarker and Imaging

这项核心建议的总体目标是建立一个安全、可靠和全面的生物安全水平- 3(BSL3)支持和增强SERCEB/RCE活性的宿主-病原体相互作用核心 调查人员。核心将提供最先进的流式细胞术和细胞分类，多模式活动物 对SERCEB或其他RCE研究人员进行成像和多重生物标记物分析 杜克大学的区域生物遏制实验室或将标本送到核心的成员调查人员 以供分析。为了实现这一目标，我们在BSL3遏制中提供了四项基本技术：BDFACS ARIA细胞分选仪、IVIS-Xenogen光谱成像仪、BibRad Luminex珠阵列读取器和 雅培细胞Dyn 3700兽用血细胞分析仪。为了支持这些基础技术，我们拥有 离线-安全壳外部的计算机工作站，专用的BSL2和BSL3实验室空间用于样品 筹备工作，在BSL3和ABSL3程序方面训练有素的技术人员，以及一名PI和联合调查员 在免疫监测研究和核心设施管理方面建立了良好的记录。 这些核心技术包含在杜克大学NIAID-RBL的BSL3中，并作为协作的核心运行 与建议的SERCEB空气生物学/动物模型核心。这将允许全面的宿主-病原体 根据SERCEB/RCE调查人员的适当标准操作程序在BSL3进行分析。这个 使用流式细胞术和活体成像技术将使我们能够监测宿主的反应和 优先病原体的感染、复制、持久性和传播过程。血液和组织 生物标记物分析将提供对宿主-病原体相互作用的进一步有价值的洞察，这只会 加强SERCEB联合体中的项目提出的挑战和机制研究。范围： 由该宿主-病原体相互作用核心支持的主动生物医学研究包括基础 优先和选择性病原体的致病性、先天免疫和获得性免疫以及宿主防御 针对已识别的生物威胁病原体的药物、诊断和疫苗的开发。

The overall objective of this core proposal is to establish a safe, secure and comprehensive Biosafety Level - 3 (BSL3) host-pathogen interaction core to support and enhance the activities of the SERCEB/RCE investigators. The core will provide state-of-the-art flow cytometry and cell sorting, multi-modal live animal imaging, and multiplex biomarker analysis for SERCEB or other RCE investigators running studies in the Regional Biocontainment Laboratory at Duke or for member investigators that send specimens to the core for analysis. To accomplish this goal we have available four base technologies in BSL3 containment: a BDFACS Aria cell sorter, an IVIS-Xenogen Spectrum imager, a BibRad luminex bead array reader and an Abbott Cell Dyn 3700 veterinary hematology analyzer. In support of these base technologies we have offline-computer workstations outside containment, dedicated BSL2 and BSL3 laboratory space for sample preparation, highly trained technical staff in BSL3 and ABSL3 procedures and a PI and Co-investigator with an established track record in immune monitoring research and core facility management. These core technologies are contained at BSL3 in the NIAID-RBL at Duke and run as a core in collaboration with the proposed SERCEB Aerobiology/Animal Models core. This will allow comprehensive host-pathogen analysis at BSL3 under appropriate Standard Operating Procedures for SERCEB/RCE investigators. The use of flow cytometry and in vivo imaging techniques will allow us to monitor both host response and the course of infection, replication, persistence, and dissemination of priority pathogens. Blood and tissue biomarker analysis will provided further valuable insight into host-pathogen interactions which will only enhance the challenge and mechanistic studies proposed by projects in the SERCEB consortium. Areas of active biomedical investigation to be supported by this host-pathogen interation core include fundamental pathogenicity of Priority and Select Agent pathogens, innate and adaptive immunity and host'defense, and development of drugs, diagnostics and vaccines for identified bio-threat pathogens.