BSL3 Flow Biomarker and Imaging

这一核心提案的总体目标是建立一个安全、可靠和全面的生物安全水平， 3（BSL 3）宿主-病原体相互作用核心，以支持和增强SERCEB/RCE的活性 investigators.核心将提供最先进的流式细胞术和细胞分选，多模式活动物 成像和多重生物标志物分析，用于SERCEB或其他RCE研究者在 杜克的区域生物防护实验室或将样本送往核心的成员研究者 用于分析为了实现这一目标，我们在BSL 3安全壳中有四种基本技术： Aria细胞分选仪、IVIS-Xenogen光谱成像仪、BibRad luminex珠粒阵列读取器和免疫分析仪。 Abbott Cell Dyn 3700兽用血液分析仪。为了支持这些基础技术， 安全壳外的离线计算机工作站，用于样品的专用BSL 2和BSL 3实验室空间 准备，BSL 3和ABSL 3程序方面训练有素的技术人员以及PI和合作研究者， 在免疫监测研究和核心设施管理方面有着良好的记录。 这些核心技术包含在杜克NIAID-RBL的BSL 3中，并作为协作核心运行 SERCEB Aerobiology/Animal Models核心。这将使全面的主机病原体 根据SERCEB/RCE研究者的适当标准操作规程在BSL 3进行分析。的 使用流式细胞术和体内成像技术将使我们能够监测宿主的反应和 感染过程、复制、持久性和优先病原体的传播。血液和组织 生物标志物分析将提供对宿主-病原体相互作用的进一步有价值的了解， 加强SERCEB联盟项目提出的挑战和机制研究。领域 由宿主-病原体相互作用核心支持的积极生物医学研究包括基本的 优先病原体和选择病原体的致病性、先天性和适应性免疫以及宿主防御，以及 针对已查明的生物威胁病原体开发药物、诊断方法和疫苗。

The overall objective of this core proposal is to establish a safe, secure and comprehensive Biosafety Level - 3 (BSL3) host-pathogen interaction core to support and enhance the activities of the SERCEB/RCE investigators. The core will provide state-of-the-art flow cytometry and cell sorting, multi-modal live animal imaging, and multiplex biomarker analysis for SERCEB or other RCE investigators running studies in the Regional Biocontainment Laboratory at Duke or for member investigators that send specimens to the core for analysis. To accomplish this goal we have available four base technologies in BSL3 containment: a BDFACS Aria cell sorter, an IVIS-Xenogen Spectrum imager, a BibRad luminex bead array reader and an Abbott Cell Dyn 3700 veterinary hematology analyzer. In support of these base technologies we have offline-computer workstations outside containment, dedicated BSL2 and BSL3 laboratory space for sample preparation, highly trained technical staff in BSL3 and ABSL3 procedures and a PI and Co-investigator with an established track record in immune monitoring research and core facility management. These core technologies are contained at BSL3 in the NIAID-RBL at Duke and run as a core in collaboration with the proposed SERCEB Aerobiology/Animal Models core. This will allow comprehensive host-pathogen analysis at BSL3 under appropriate Standard Operating Procedures for SERCEB/RCE investigators. The use of flow cytometry and in vivo imaging techniques will allow us to monitor both host response and the course of infection, replication, persistence, and dissemination of priority pathogens. Blood and tissue biomarker analysis will provided further valuable insight into host-pathogen interactions which will only enhance the challenge and mechanistic studies proposed by projects in the SERCEB consortium. Areas of active biomedical investigation to be supported by this host-pathogen interation core include fundamental pathogenicity of Priority and Select Agent pathogens, innate and adaptive immunity and host'defense, and development of drugs, diagnostics and vaccines for identified bio-threat pathogens.