Immune Monitoring

RadCCORE的免疫监测核心由杜克人类疫苗研究所(DHVI)内的两个服务实验室提供的技术和服务组成：流式细胞仪设施和免疫重建和生物标记设施。RadCCORE免疫监测核心(核心C)将定期为研究人员提供高质量、最先进的细胞分类、多重蛋白质阵列和T细胞受体基因表达分析，用于基础和应用研究工作。续订中提供的服务反映了第1-5年中核心C的实际利用率。免疫监测核心的优势在于：1)提供先进的多色荧光激活细胞分选；2)使用BioPlex微珠阵列阅读器(BioRad)对生物样本，如组织培养上清、血清/血浆和肺灌洗液等进行有针对性的多重蛋白质阵列分析；3)通过T细胞受体切除环量化和外周T细胞受体β可变基因利用的谱系分析，对小鼠、人类和猴子的T细胞免疫重建进行监测。通过与RadCCORE科学项目用户群的教育和合作，我们将开发和优化他们的具体分析方法，并更有效地利用免疫监测核心提供的最先进的仪器。对我们所有这三个目标至关重要的是致力于持续开发新的分析方法，以预测和满足RadCCORE调查人员的未来需求。

The Immune Monitoring Core for RadCCORE is comprised of technologies and services offered by two service laboratories within the Duke Human Vaccine Institute (DHVI): Flow Cytometry Facility, and Immune Reconstitution and Biomarker Facility. The RadCCORE Immune Monitoring Core (Core C) will routinely provide investigators with high quality, state-of-the-art cell sorting, multiplex protein arrays, and T cell receptor gene expression analysis for basic and applied research efforts. Services offered in the renewal reflect actual Core C utilization in years 1-5. Alms of the Immune Monitoring Core are: 1) Provide state-of the-art, multi-color, fluorescence activated cell sorting; 2) Provide targeted multiplex protein array profiling of biological samples, such as tissue culture supernatant, serum/plasma, and lung lavage fluid, using our BioPlex bead array reader (BioRad); and 3) Provide T cell immune reconstitution monitoring in mice, humans and monkeys by performing T cell receptor excision circle quantification and repertoire analysis of peripheral T cell receptor beta variable gene utilization. By educating and working in collaboration with the RadCCORE scientific program user base we will develop and optimize their specific assays and more effectively utilize the state-of-the-art instrumentation offered by the Immune Monitoring Core. Critical to all three of our aims is a commitment to ongoing development of new assays to anticipate and meet the future needs of the RadCCORE investigators.