Immune Monitoring

radcoore的免疫监测核心由杜克大学人类疫苗研究所（DHVI）的两个服务实验室提供的技术和服务组成：流式细胞仪设施和免疫重建和生物标志物设施。radcoore免疫监测核心（Core C）将为研究人员提供高质量、最先进的细胞分选、多重蛋白阵列和T细胞受体基因表达分析，用于基础和应用研究工作。续订中提供的服务反映了第1-5年核心C的实际使用情况。免疫监测核心的施舍是：1)提供最先进的，多色，荧光激活的细胞分选；2)使用我们的BioPlex头阵列读取器（BioRad），对生物样品（如组织培养上清、血清/血浆和肺灌洗液）提供靶向多重蛋白阵列分析；3)通过T细胞受体切除环定量和外周血T细胞受体β可变基因利用库分析，对小鼠、人类和猴子进行T细胞免疫重建监测。通过教育和与radcoore科学计划用户群的合作，我们将开发和优化他们的特定分析，并更有效地利用免疫监测核心提供的最先进的仪器。我们这三个目标的关键是致力于不断开发新的检测方法，以预测和满足radcore研究人员的未来需求。

The Immune Monitoring Core for RadCCORE is comprised of technologies and services offered by two service laboratories within the Duke Human Vaccine Institute (DHVI): Flow Cytometry Facility, and Immune Reconstitution and Biomarker Facility. The RadCCORE Immune Monitoring Core (Core C) will routinely provide investigators with high quality, state-of-the-art cell sorting, multiplex protein arrays, and T cell receptor gene expression analysis for basic and applied research efforts. Services offered in the renewal reflect actual Core C utilization in years 1-5. Alms of the Immune Monitoring Core are: 1) Provide state-of the-art, multi-color, fluorescence activated cell sorting; 2) Provide targeted multiplex protein array profiling of biological samples, such as tissue culture supernatant, serum/plasma, and lung lavage fluid, using our BioPlex bead array reader (BioRad); and 3) Provide T cell immune reconstitution monitoring in mice, humans and monkeys by performing T cell receptor excision circle quantification and repertoire analysis of peripheral T cell receptor beta variable gene utilization. By educating and working in collaboration with the RadCCORE scientific program user base we will develop and optimize their specific assays and more effectively utilize the state-of-the-art instrumentation offered by the Immune Monitoring Core. Critical to all three of our aims is a commitment to ongoing development of new assays to anticipate and meet the future needs of the RadCCORE investigators.