CORE--ANIMAL PRODUCTION, RATS

核心——动物生产，大鼠

• 批准号: 6712896
• 负责人: TING-KAI K LI
• 金额: $ 37.21万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6712896
• 关键词: alcoholic beverage consumption; alcoholism /alcohol abuse; animal breeding; animal colony; biomedical facility; laboratory rat

DESCRIPTION (provided by applicant): The Animal Production Core (A_PC) will continue to supply selectively bred and inbred P/NP (alcohol-preferring and -nonpreferring), HAD1/LAD 1, and HAD2/LAD2 (replicate high and low alcohol drinking) rats for research. These rats will be provided to researchers supported by or affiliated with our ARC (many are funded by R01 s and K awards, and their research is dependent on these selectively bred or inbred rats), and to collaborating/independent researchers at other Institutions (some also have active NIAAA grants that require our selectively bred and inbred rats). The specific aims are: a) To continue to produce selectively bred P/NP rats for research use. b) To continue to produce HAD1/LAD1 and HAD2/LAD2 rats for research use, and c) To continue inbreeding ofP/NP, HAD1/LAD1 and HAD2/LAD2 rats. Additionally, although not budgeted, we would like to continue to assist in the creation of reciprocal congenic lines from inbred P/NP strains in order to narrow the critical region of Chr 4 QTL for alcohol preference, i.e., the QTL with a led score of 9.2, found in F2s from an iP x iNP (inbred P and NP) cross. Investigators will have a choice to use either selectively bred P/NP, HAD 1/LAD 1 and HAD2/LAD2 lines or their respective inbred strains. However, the APC would like to encourage efforts toward better phenotypic characterization of the iP and iNP strains, particularly strains iP5c and iP 10a because these inbred strains have been extensively genotyped and congenic lines are being raised. Additionally, genotypic and phenotypic characterizations of specific iliAD 1/iLAD 1 and iHAD2/iLAD2 strains will be encouraged. This is because inbred strains are isogenic constants (genotypically and phenotypically) and data collected from them are cumulative. Crosses can then be made from these inbred strains to synthesize various populations of desired specific characteristics (e.g., generation of F2 progeny and from them, conducting replicate short-term selective breeding for genetic correlative studies).

描述（由申请方提供）：动物生产核心（A_PC）将继续提供 选择性育种和近交P/NP（酒精偏好和非酒精偏好），HAD 1/LAD 1，以及 HAD 2/LAD 2（重复高和低酒精饮用）大鼠用于研究。这些大鼠将提供给由我们的ARC支持或隶属于我们的ARC的研究人员（许多人由R 01和K奖资助，他们的研究依赖于这些选择性繁殖或近交的大鼠），以及其他机构的合作/独立研究人员（有些也有积极的NIAAA赠款，需要我们的选择性繁殖和近交的大鼠）。具体目标是：a）继续生产选择性繁殖的P/NP大鼠用于研究。B）继续生产供研究使用的HAD 1/LAD 1和HAD 2/LAD 2大鼠;和c）继续近交P/NP、HAD 1/LAD 1和HAD 2/LAD 2大鼠。此外，尽管没有预算，我们希望继续协助从近交P/NP品系创建相互同源系，以缩小Chr 4 QTL对酒精偏好的关键区域，即，在来自iP × iNP（近交系P和NP）杂交F2中发现的具有9.2的led评分的QTL。研究者可以选择使用选择性育种的P/NP、HAD 1/LAD 1和HAD 2/LAD 2品系或其各自的近交系。 然而，APC希望鼓励对iP和iNP菌株，特别是菌株iP 5c和iP 10a进行更好的表型表征，因为这些近交系已经被广泛地基因分型，并且正在培育同源系。此外，将鼓励对特定的iiAD 1/iLAD 1和iHAD 2/iLAD 2菌株进行基因型和表型表征。这是因为近交系是等基因常数（基因型和表型），从它们收集的数据是累积的。然后可以从这些近交系进行杂交以合成具有所需特定特征的各种群体（例如，F_2代，并从它们进行重复的短期选择育种，用于遗传相关性研究）。