CORE--ANIMAL PRODUCTION, RATS

核心——动物生产，大鼠

• 批准号: 6712896
• 负责人: TING-KAI K LI
• 金额: $ 37.21万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6712896
• 关键词: alcoholic beverage consumption; alcoholism /alcohol abuse; animal breeding; animal colony; biomedical facility; laboratory rat

DESCRIPTION (provided by applicant): The Animal Production Core (A_PC) will continue to supply selectively bred and inbred P/NP (alcohol-preferring and -nonpreferring), HAD1/LAD 1, and HAD2/LAD2 (replicate high and low alcohol drinking) rats for research. These rats will be provided to researchers supported by or affiliated with our ARC (many are funded by R01 s and K awards, and their research is dependent on these selectively bred or inbred rats), and to collaborating/independent researchers at other Institutions (some also have active NIAAA grants that require our selectively bred and inbred rats). The specific aims are: a) To continue to produce selectively bred P/NP rats for research use. b) To continue to produce HAD1/LAD1 and HAD2/LAD2 rats for research use, and c) To continue inbreeding ofP/NP, HAD1/LAD1 and HAD2/LAD2 rats. Additionally, although not budgeted, we would like to continue to assist in the creation of reciprocal congenic lines from inbred P/NP strains in order to narrow the critical region of Chr 4 QTL for alcohol preference, i.e., the QTL with a led score of 9.2, found in F2s from an iP x iNP (inbred P and NP) cross. Investigators will have a choice to use either selectively bred P/NP, HAD 1/LAD 1 and HAD2/LAD2 lines or their respective inbred strains. However, the APC would like to encourage efforts toward better phenotypic characterization of the iP and iNP strains, particularly strains iP5c and iP 10a because these inbred strains have been extensively genotyped and congenic lines are being raised. Additionally, genotypic and phenotypic characterizations of specific iliAD 1/iLAD 1 and iHAD2/iLAD2 strains will be encouraged. This is because inbred strains are isogenic constants (genotypically and phenotypically) and data collected from them are cumulative. Crosses can then be made from these inbred strains to synthesize various populations of desired specific characteristics (e.g., generation of F2 progeny and from them, conducting replicate short-term selective breeding for genetic correlative studies).

描述(申请人提供)：动物生产核心(A_PC)将继续提供 选择性繁殖和近交系P/NP(偏爱酒精和不偏爱酒精)、HAD1/LAD 1，和 HAD2/LAD2(复制高、低度饮酒)大鼠为研究对象。这些大鼠将被提供给由我们ARC支持或附属于我们ARC的研究人员(许多是由R01 S和K奖资助的，他们的研究依赖于这些选择性繁殖或近交系大鼠)，以及其他机构的合作/独立研究人员(一些机构也有NIAAA的有效资助，需要我们选择性地培育和近交系繁殖的大鼠)。具体目标是：a)继续培育用于研究的选择性繁殖的P/NP大鼠。B)继续培育用于研究的HAD1/LAD1和HAD2/LAD2大鼠；c)继续近亲交配OFP/NP、HAD1/LAD1和HAD2/LAD2大鼠。此外，虽然没有预算，但我们希望继续帮助从自交系P/NP品系中创建互作同源系，以缩小Chr 4对酒精偏好的QTL的临界区域，即在IP×INP(自交系P和NP)杂交的F2s中发现的LED得分为9.2的QTL。研究人员将可以选择使用选择性培育的P/NP、HAD 1/LAD 1和HAD2/LAD2品系或它们各自的近交系。 然而，APC希望鼓励对IP和INP菌株，特别是菌株IP5c和IP 10a进行更好的表型表征，因为这些近交系已经进行了广泛的基因分型，并且正在培育同源品系。此外，还将鼓励对特定的Iliad1/iLAD1和iHAD2/iLAD2菌株进行基因分型和表型鉴定。这是因为近交系是等基因常数(基因和表型)，从它们收集的数据是累积的。然后，可以用这些近交系进行杂交，以合成具有所需特定特性的各种群体(例如，产生F2后代并从中进行重复的短期选择育种以进行遗传相关性研究)。